A cell line producing recombinant nerve growth factor evokes growth responses in intrinsic and grafted central cholinergic neurons.
A cell line producing recombinant nerve growth factor evokes growth responses in intrinsic and grafted central cholinergic neurons.
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产生重组神经生长因子的细胞系可引起内在和移植的中枢胆碱能神经元的生长反应。
DOI：
10.1073/pnas.86.12.4756
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发表时间：
1989
影响因子：
11.1
通讯作者：
Persson,H
中科院分区：
文献类型：
--
作者：
Ernfors,P;Ebendal,T;Olson,L;Mouton,P;Strömberg,I;Persson,H
将大鼠β神经生长因子（NGF）基因插入哺乳动物表达载体，并与具有新霉素抗性的质粒共转染小鼠3T3成纤维细胞。从这种转染中选择了一种稳定的细胞系，它含有几百个大鼠NGF基因拷贝，并产生过量的重组NGF。这些基因修饰的细胞被植入大鼠大脑，作为神经生长因子对中枢神经系统神经元体内作用的探针。在阿尔茨海默病皮质胆碱能缺陷的模型中，我们证明了胎儿基底前脑胆碱能神经元移植物的存活率和纤维生长的显著增加，以及大脑皮层中完整的成人固有胆碱能回路对纤维形成的刺激。完整纹状体的成体胆碱能中间神经元也向植入的成纤维细胞大力萌芽。我们的研究结果表明，该模型对神经退行性疾病的未来治疗具有启示意义。
The rat beta nerve growth factor (NGF) gene was inserted into a mammalian expression vector and cotransfected with a plasmid conferring resistance to neomycin into mouse 3T3 fibroblasts. From this transfection a stable cell line was selected that contains several hundred copies of the rat NGF gene and produces excess levels of recombinant NGF. Such genetically modified cells were implanted into the rat brain as a probe for in vivo effects of NGF on central nervous system neurons. In a model of the cortical cholinergic deficits in Alzheimer disease, we demonstrate a marked increase in the survival of, and fiber outgrowth from, grafts of fetal basal forebrain cholinergic neurons, as well as stimulation of fiber formation by intact adult intrinsic cholinergic circuits in the cerebral cortex. Adult cholinergic interneurons in intact striatum also sprout vigorously toward implanted fibroblasts. Our results suggest that this model has implications for future treatment of neurodegenerative diseases.
DOI：
10.1016/0169-328x(89)90011-9
发表时间：
1989-01-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
GOEDERT, M;FINE, A;CHAO, MV
通讯作者：
CHAO, MV
观察百岁老人的大脑
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
Meng-Tsen Ke;Satoshi Fujimoto;Takeshi Imai;石内 勝吾
通讯作者：
石内 勝吾