Morphological and mechanical characteristics of the reconstructed rat abdominal wall following use of a wet electrospun biodegradable polyurethane elastomer scaffold.
Morphological and mechanical characteristics of the reconstructed rat abdominal wall following use of a wet electrospun biodegradable polyurethane elastomer scaffold.
复制标题
DOI：
10.1016/j.biomaterials.2010.01.051
复制
发表时间：
2010-04
期刊：
影响因子：
14
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Hashizume, Ryotaro;Fujimoto, Kazuro L.;Hong, Yi;Amoroso, Nicholas J.;Tobita, Kimimasa;Miki, Toshio;Keller, Bradley B.;Sacks, Michael S.;Wagner, William R.
尽管目前用于腹壁修复的材料多种多样，但常见的并发症包括疝形成、感染以及与天然组织的力学不匹配。一种可降解合成材料最终被力学性能接近天然状态的组织所替代的方法可能会避免这些并发症。我们在此报道使用湿电纺技术制备用于腹壁替代的可生物降解支架，在该技术中，可生物降解弹性体聚（酯氨酯）脲（PEUU）的纤维与电喷雾的血清培养基同时沉积。研究发现，湿电纺PEUU（wet ePEUU）与干法加工的ePEUU相比，表现出明显不同的力学行为，并且微观结构也发生了改变。在大鼠腹壁替代模型中，湿ePEUU支架（1×2.5厘米）在8周时呈现出接近生理力学行为的愈合结果。观察到大量具有收缩平滑肌标志物的细胞浸润以及大量细胞外基质（胶原蛋白、弹性蛋白）的形成。干法ePEUU和膨体聚四氟乙烯的对照植入物没有明显的细胞浸润，也没有呈现出大鼠腹壁的天然力学各向异性。这些结果表明，这种新报道的湿电纺工艺在体内表现出明显不同的行为，为一种日益常见的生物材料加工技术提供了一种可能有用的改进。
Although a variety of materials are currently used for abdominal wall repair, general complications encountered include herniation, infection, and mechanical mismatch with native tissue. An approach wherein a degradable synthetic material is ultimately replaced by tissue mechanically approximating the native state could obviate these complications. We report here on the generation of biodegradable scaffolds for abdominal wall replacement using a wet electrospinning technique in which fibers of a biodegradable elastomer, poly(ester urethane)urea (PEUU), were concurrently deposited with electrosprayed serum-based culture medium. Wet electrospun PEUU (wet ePEUU) was found to exhibit markedly different mechanical behavior and to possess an altered microstructure relative to dry processed ePEUU. In a rat model for abdominal wall replacement, wet ePEUU scaffolds (1 × 2.5 cm) provided a healing result that developed toward approximating physiologic mechanical behavior at 8 wks. An extensive cellular infiltrate possessing contractile smooth muscle markers was observed together with extensive extracellular matrix (collagens, elastin) elaboration. Control implants of dry ePEUU and expanded polytetrafluoroethylene did not experience substantial cellular infiltration and did not take on the native mechanical anisotropy of the rat abdominal wall. These results illustrate the markedly different in vivo behavior observed with this newly reported wet electrospinning process, offering a potentially useful refinement of an increasingly common biomaterial processing technique.
登录
查看更多内容
影响因子：
9
作者：
Jernigan, TW;Fabian, TC;Bee, TK
通讯作者：
Bee, TK
影响因子：
2.3
作者：
Ott, R.;Hartwig, T.;Koeckerling, F.
通讯作者：
Koeckerling, F.
DOI：
10.1002/jbm.b.31234
发表时间：
2009-05-01
影响因子：
3.4
作者：
Bellon, J. M.;Rodriguez, M.;Bujan, J.
通讯作者：
Bujan, J.
DOI：
10.1002/jbm.10204
发表时间：
2002-09-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Guan, JJ;Sacks, MS;Wagner, WR
通讯作者：
Wagner, WR
影响因子：
4.3
作者：
Bir, Shyamal Chandra;Esaki, Jiro;Sakata, Ryuzo
通讯作者：
Sakata, Ryuzo