Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis.
Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis.
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DOI：
10.1016/j.cub.2010.12.013
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发表时间：
2011-01-25
期刊：
影响因子：
9.2
通讯作者：
Millar, Andrew J.
中科院分区：
文献类型：
--
作者：
Dixon, Laura E.;Knox, Kirsten;Kozma-Bognar, Laszlo;Southern, Megan M.;Pokhilko, Alexandra;Millar, Andrew J.
生物钟为真核生物提供了强大的24小时生物节律。植物中的时钟基因回路包括互锁的转录反馈环，如中所述，其中早晨表达的转录因子昼夜节律时钟相关1（CCA 1）和晚伸长下胚轴（LHY）抑制晚上基因的表达，特别是CAB表达的时间安排1（TOC 1）。早花蛋白3（ELF 3）被认为是光信号传导到生物钟的抑制因子，矛盾的是，它是光诱导基因CCA 1和LHY的激活因子。我们使用cca 1 -11 lhy-21 elf 3 -4植物将ELF 3的阻遏功能与其下游靶标CCA 1和LHY分离。我们进一步证明，ELF 3协会与假反应调节因子9（PRR 9），一个抑制剂的CCA 1和LHY的表达，在一个时间依赖性的方式启动子物理。因此，ELF 3的抑制功能与LHY和CCA 1的间接激活一致，通过直接ELF 3靶点PRR 9进行双负连接。这种机制协调了ELF 3在夜间时钟网络中的功能，并指出了ELF 3在白天的进一步影响。ELF 3是TOC 1、PRR 9、GI和PRR 7基因表达的调节因子。ELF 3的抑制与LHY和CCA 1的抑制在遗传上是分离的。ELF 3以时间依赖性方式与PRR 9的启动子物理缔合。
The circadian clock provides robust, ∼24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in, whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock and, paradoxically, as an activator of the light-induced genes CCA1 and LHY. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day. ► ELF3 is a regulator of TOC1, PRR9, GI, and PRR7 gene expression ► Repression by ELF3 is genetically separable from repression by LHY and CCA1 ► ELF3 physically associates with the promoter of PRR9 in a time-dependent manner
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