Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.
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三维胶原支架对胶质瘤细胞表达谱及生物学功能的影响
DOI：
10.3892/ijo.2018.4330
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发表时间：
2018-06
影响因子：
5.2
通讯作者：
Ren C
中科院分区：
文献类型：
--
作者：
Jia W;Jiang X;Liu W;Wang L;Zhu B;Zhu H;Liu X;Zhong M;Xie D;Huang W;Jia W;Li S;Liu X;Zuo X;Cheng D;Dai J;Ren C
三维（3D）培养越来越多地用于研究肿瘤细胞生物学，以改进对自然发育环境的模拟。然而，3D培养如何影响胶质瘤细胞的基因表达和生物学功能仍有待充分阐明。本研究采用不同孔径的胶原支架构建三维培养环境，比较U87、U251、HS683等胶质瘤细胞在三维胶原支架中的基因表达谱及相关生物学功能与常规二维培养细胞的差异。最后，研究了调节这些差异的可能信号通路。结果发现，3D胶原支架培养可上调与干细胞干性、细胞周期、细胞凋亡、上皮-间质转化、迁移、侵袭及胶质瘤恶性相关基因的表达，并诱导相应的功能改变。凋亡通路Wnt通路、Sonic Hedgehog通路和Notch通路可能参与了这些变化的调控。胶原支架的孔径大小似乎不影响胶质瘤细胞的基因表达或功能。本研究结果提示，3D胶原支架增强了胶质瘤细胞的恶性，可能是一种有前景的胶质瘤体外研究平台。
Three-dimensional (3D) culture has been increasingly used to investigate tumor cell biology for improved simulation of the natural developing environment. However, the way in which 3D culture affects the gene expression and biological functions of glioma cells remains to be fully elucidated. In the present study, 3D culture environments were established using collagen scaffolds with different pore sizes, followed by the comparison of gene expression profiles and associated biological functions of glioma cells, including the U87, U251 and HS683 cell lines, in 3D collagen scaffolds with conventional two-dimensional (2D) cultured cells. Finally, the possible signaling pathways regulating these differences were investigated. It was found that the 3D collagen scaffold culture upregulated the expression of genes associated with stemness, cell cycle, apoptosis, epithelia-mesenchymal transition, migration, invasion and glioma malignancy, and induced the corresponding functional changes. Apoptotic pathways, the Wnt pathway, Sonic Hedgehog pathway and Notch pathway, may be involved in the regulation of these changes. The aperture size of the collagen-scaffold did not appear to affect the gene expression or functions of the glioma cells. The results of the study suggested that the 3D collagen scaffold enhanced the malignancy of glioma cells and may be a promising in vitro platform for investigations of glioma.
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影响因子：
11.2
作者：
Borcherding N;Kusner D;Kolb R;Xie Q;Li W;Yuan F;Velez G;Askeland R;Weigel RJ;Zhang W
通讯作者：
Zhang W
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
5.8
作者：
Du, Mingchun;Liang, Hui;Dai, Jianwu
通讯作者：
Dai, Jianwu
影响因子：
14
作者：
Heffernan JM;McNamara JB;Borwege S;Vernon BL;Sanai N;Mehta S;Sirianni RW
通讯作者：
Sirianni RW
DOI：
10.1039/c0ib00142b
发表时间：
2011-05
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Fallica B;Makin G;Zaman MH
通讯作者：
Zaman MH