An integrated microfluidic platform for in situ cellular cytokine secretion immunophenotyping.
An integrated microfluidic platform for in situ cellular cytokine secretion immunophenotyping.
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DOI：
10.1039/c2lc40619e
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发表时间：
2012-10-21
期刊：
影响因子：
6.1
通讯作者：
Kurabayashi K
中科院分区：
文献类型：
--
作者：
Huang NT;Chen W;Oh BR;Cornell TT;Shanley TP;Fu J;Kurabayashi K
快速，定量检测细胞分泌的生物标志物蛋白与低样本量具有很大的希望，以推进细胞免疫表型技术的个性化诊断和治疗传染病。在这里，我们用THP-1人类急性单核细胞白血病细胞系（人类单核细胞模型）实现了这样的实验，使用高度集成的微流控平台，结合免洗珠基化学发光免疫检测方案。我们的微流体装置使我们能够在一个控制良好的芯片环境下，用脂多糖（LPS）刺激细胞，脂多糖是一种内毒素，由于人体严重的免疫反应而导致脓毒性休克。随后在设备内测量受刺激的THP-1细胞分泌的肿瘤坏死因子-α (TNF-α)，不需要冲洗过程。我们的研究获得了高灵敏度的细胞免疫表型，比目前的细胞刺激试验少20倍。总检测时间也比常规酶联免疫吸附法（ELISA）短7倍。我们使用微流控平台原位监测免疫细胞功能的策略可以通过实现快速，样本高效的细胞免疫表型分析，影响未来急性传染病和免疫疾病的医学治疗。
Rapid, quantitative detection of cell-secreted biomarker proteins with a low sample volume holds great promise to advance cellular immunophenotyping techniques for personalized diagnosis and treatment of infectious diseases. Here we achieved such an assay with the THP-1 human acute moncytic leukemia cell line (a model for human monocyte) using a highly integrated microfluidic platform incorporating a no-wash bead-based chemiluminescence immunodetection scheme. Our microfluidic device allowed us to stimulate cells with lipopolysaccharide (LPS), which is an endotoxin causing septic shock due to severely pronounced immune response of the human body, under a well-controlled on-chip environment. Tumor necrosis factor-alpha (TNF-α) secreted from stimulated THP-1 cells was subsequently measured within the device with no flushing process required. Our study achieved high-sensitivity cellular immunophenotyping with 20-fold fewer cells than current cell-stimulation assay. The total assay time was also 7 times shorter than that of a conventional enzyme-linked immunosorbent assay (ELISA). Our strategy of monitoring immune cell functions in situ using a microfluidic platform could impact future medical treatments of acute infectious diseases and immune disorders by enabling a rapid, sample-efficient cellular immunophenotyping analysis.
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DOI：
10.1186/cc5055
发表时间：
2006
期刊：
Critical care (London, England)
影响因子：
--
作者：
Cavaillon JM;Adib-Conquy M
通讯作者：
Adib-Conquy M
影响因子：
120.7
作者：
Boomer, Jonathan S.;To, Kathleen;Chang, Kathy C.;Takasu, Osamu;Osborne, Dale F.;Walton, Andrew H.;Bricker, Traci L.;Jarman, Stephen D., II;Kreisel, Daniel;Krupnick, Alexander S.;Srivastava, Anil;Swanson, Paul E.;Green, Jonathan M.;Hotchkiss, Richard S.
通讯作者：
Hotchkiss, Richard S.
影响因子：
2.2
作者：
Guerkov, REM;Targoni, OS;Schwander, SK
通讯作者：
Schwander, SK
影响因子：
46.9
作者：
Love, J. Christopher;Ronan, Jehnna L.;Ploegh, Hidde L.
通讯作者：
Ploegh, Hidde L.
影响因子：
8
作者：
Azizia, Mallika;Lloyd, Jillian;Peebles, Donald
通讯作者：
Peebles, Donald