A novel technique for the study of bacterial cell mechanical properties
A novel technique for the study of bacterial cell mechanical properties
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研究细菌细胞机械特性的新技术
DOI：
10.1023/a:1008919915047
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发表时间：
1999
影响因子：
--
通讯作者：
Colin R. Thomas
中科院分区：
文献类型：
--
作者：
C. Shiu;Zhibing Zhang;Colin R. Thomas
描述了一种用于测量细菌的破裂力并将其与细胞大小相关联的显微操作方法。在6.2 μm s-1的压缩速度下，来自分批培养的快速生长的表皮葡萄球菌的三个样品的破裂力在3至34 μN之间变化，平均值为13.8 μN（标准误差0.8 μN）。以0.5 h−1的比生长速率在连续培养中生长的大肠杆菌具有1至9 μN的爆裂力，平均值为3.6 μN（标准误差0.4 μN）。在挤压-保持实验中，观察到力松弛，这归因于细胞失水或粘弹性或两者。在高压缩速度下，例如6.2 μm s−1，这种弛豫可以忽略不计。显微操作强度的测量可用于研究细胞的机械破碎和细胞强度对细胞生理的依赖性。
A micromanipulation method is described for measuring the bursting forces of bacteria and relating them to cell size. At a compression speed of 6.2 μm s−1, bursting forces of three samples of rapidly growing Staphylococcus epidermis from a batch culture varied from 3 to 34 μN with an average value of 13.8 μN (standard error 0.8 μN). Escherichia coli grown in continuous culture at a specific growth rate of 0.5 h−1 had bursting forces varying from 1 to 9 μN with an average value of 3.6 μN (standard error 0.4 μN). In squeeze-hold experiments, force relaxation was observed, which was attributed to water loss from the cells, or viscoelasticity, or both. At high compression speed, such as 6.2 μm s−1, this relaxation could be neglected. Micromanipulation strength measurements might be used in studies of cell mechanical disruption and of the dependence of cell strength on cell physiology.
微量移液器技术在测量培养牛主动脉内皮细胞力学性能中的应用。
DOI：
10.1115/1.3138638
发表时间：
1987
期刊：
Journal of biomechanical engineering
影响因子：
--
作者：
Sato,M;Levesque,MJ;Nerem,RM
通讯作者：
Nerem,RM