AUGMENTATION OF THE ANTIBODY RESPONSE BY HAPTEN HELP

The purpose of the present work was to characterize the immune response (Ir) genes that influence augmentation of the antibody response by help with the hapten azobenzene‐arsonate (ABA). Hapten help was measured as the augmentation in the plaque‐forming cell (PFC) response to bovine gamma gloulin (BGG) after priming mice with ABA conjugated to ovalbumin (OVA) and challenging subsequently with ABA‐BGG. The first approach involved inbred mouse strains that were matched for H‐2 but differed in their non‐H‐2 genetic backgrounds. B10.D2 mice were low responders even though they shared the H‐2d haplotype with BALB/c and DBA/2 mice, which were high responders. C57BL/10 mice were high responders even though they shared the H‐2b haplotype with C57BL/6, C3H.SW, and A.BY mice, all of which were strains that were low responders. The second approach was to identify any segregation of H‐2 woth the gene(s) encoding susceptibility to hapten help in the backcross generation. (BALB/c x C57BL/6) F1 hybrids were backcrossed to C57BL/6. The results showed no association with the major histocompatibility complex (MHC).

本研究的目的是对免疫应答（Ir）基因进行特征描述，这些基因通过对半抗原偶氮苯砷酸盐（ABA）的辅助作用影响抗体应答的增强。半抗原辅助作用通过用与卵清蛋白（OVA）偶联的ABA对小鼠进行初次免疫，随后用ABA - BGG进行攻击后，对牛丙种球蛋白（BGG）的空斑形成细胞（PFC）应答的增强来衡量。第一种方法涉及近交系小鼠品系，它们的H - 2相同，但非H - 2遗传背景不同。B10.D2小鼠是低应答者，尽管它们与高应答者BALB/c和DBA/2小鼠具有相同的H - 2d单倍型。C57BL/10小鼠是高应答者，尽管它们与低应答者C57BL/6、C3H.SW和A.BY小鼠具有相同的H - 2b单倍型。第二种方法是在回交一代中鉴定H - 2与编码对半抗原辅助敏感的基因的任何分离情况。（BALB/c×C57BL/6）F1杂种与C57BL/6回交。结果显示与主要组织相容性复合体（MHC）无关联。