Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes
Optical waveguide for common path simultaneous refractive index and broadband absorption measurements in small volumes
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用于小体积共路径同时折射率和宽带吸收测量的光波导
DOI：
10.1016/j.snb.2016.07.162
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Gupta R
中科院分区：
文献类型：
--
作者：
Gupta R
使用单一光源和探测器进行测量时，同时进行小体积折射率监测和吸收光谱是有益的，但执行起来具有挑战性。为此，这项工作提出了一种由沉积在玻璃上的多孔波导组成的光学器件，称为染料掺杂漏波导（DDLW）。波导由掺杂有染料活性蓝 4 的琼脂糖制成。甘油和罗丹明 6G 用作示例系统，以证明 DDLW 在 3.5 nL 的小公共体积中同时进行折射率和宽带吸收测量的可行性。在这种情况下，固定的活性蓝4允许波导的谐振角可视化以用于折射率监测，而游离罗丹明6G引起的附加吸收通过测量DDLW的反射率分布中与波长相关的附加损耗来确定。 DDLW 的折射率灵敏度和检测限分别为 106.32 ± 0.97° RIU−1 和 2.82 × 10−6。通过结合这两种测量，DDLW 被证明适合监测罗丹明 6G 的吸收光谱和反常色散以及其与活性蓝 4 相互作用的性质。这些相互作用导致波导中罗丹明 6G 的浓度提高了 119 到 191 倍。DDLW 被证明适合获得浓度低至 1 μM 的罗丹明 6G 的吸收光谱。未来的工作将集中于应用 DDLW 来表征分析物/确定具有生物学意义的参数，例如每个血清铁蛋白分子的铁载量。
Simultaneous refractive index monitoring and absorption spectroscopy in small volumes common to both measurements using single light source and detector are beneficial, but challenging to perform. This work presents an optical device consisting of a porous waveguide deposited on glass, which is called dye-doped leaky waveguide (DDLW), for this purpose. The waveguide was made of agarose doped with a dye, reactive blue 4. Glycerol and rhodamine 6G were used as an exemplar system to demonstrate the feasibility of DDLW to perform simultaneous refractive index and broadband absorption measurements in a small common volume of 3.5 nL. In this case, the immobilised reactive blue 4 permits visualisation of the resonance angle of the waveguide for refractive index monitoring, while the additional absorption caused by the free rhodamine 6G was determined by measuring the wavelength dependent additional losses in the reflectivity profile of the DDLW. The refractive index sensitivity and limit of detection of the DDLW was 106.32 ± 0.97° RIU−1and 2.82 × 10−6respectively. By combining the two measurements, the DDLW was shown to be suitable to monitor the absorption spectrum and anomalous dispersion of rhodamine 6G as well as the nature of its interactions with reactive blue 4. These interactions resulted in enhancing the concentration of the rhodamine 6G in the waveguide by a factor between 119 and 191. The DDLW was shown to be suitable to obtain the absorption spectrum of rhodamine 6G at concentrations as low as 1 μM. Future work will focus on the application of the DDLW to characterise analytes/determine parameters of biological significance such as iron loading per molecule of serum ferritin.
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
6.7
作者：
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DOI：
10.3390/s140917008
发表时间：
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期刊：
Sensors (Basel, Switzerland)
影响因子：
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作者：
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DOI：
10.1063/1.2894307
发表时间：
2008
期刊：
The Review of scientific instruments
影响因子：
--
作者：
S. L. Westcott;Jiangquan Zhang;R. Shelton;N. Bruce;Sachin Gupta;Steven L Keen;Jerry W. Tillman;L. Wald;B. N. Strecker;A. Rosenberger;R. Davidson;Wei Chen;K. Donovan;J. Hryniewicz
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