Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus.
Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus.
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DOI：
10.1073/pnas.1718284115
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发表时间：
2018-01-23
影响因子：
11.1
通讯作者：
Leal WS
中科院分区：
文献类型：
--
作者：
Choo YM;Xu P;Hwang JK;Zeng F;Tan K;Bhagavathy G;Chauhan KR;Leal WS
监测蚊子种群对于确定干预的最佳时机以及监测新的和以前发现的病原体的传播至关重要。诱捕怀孕的雌性更有效，因为它们比没有怀孕的雌性更容易被感染。然而，迫切需要更好的重力陷阱引诱剂来取代发酵配方，发酵配方繁琐，质量控制差，产生难闻的气味，并且不能提供长期和一致的引诱剂来源。通过使用基于南方家蚊气味受体的反向化学生态学方法，我们已经确定，大剂量的乙醛既能吸引怀孕的雌蚊，又能刺激它们在产卵盘中产卵。信息素和其他半化学物质在当今虫害和病媒综合管理战略中发挥着至关重要的作用。这些半化学物质通常是通过生物测定指导方法发现的。在这里，我们应用了一种反向化学生态学方法；也就是说，我们用嗅觉蛋白来引导我们找到假定的半化学物质。具体来说，我们使用了在致倦库蚊触角中表达最多的前10个气味受体（ORs）中的7个，这些受体尚未去孤儿化。我们在爪蟾卵母细胞记录系统中表达了这些受体，并用230种气味剂（包括生理和行为活性化合物）挑战它们。其中6个ORs是沉默的，要么是因为它们不起作用，要么是因为缺少了一种关键的气味。CquiOR36在雌性触角中显示出所有OR基因的最高转录水平，对测试面板中的所有气味都沉默，但当意外地用乙醇中的旧壬醛样品挑战时，它产生了强烈的反应。在确认新鲜样品是无活性的，并通过对旧nonanal样品中所有可能的“污染物”的仔细调查后，我们确定了活性配体为乙醛。通过携带CquiOR36基因的果蝇的触角电图记录，进一步证实了乙醛正在激活CquiOR36。雌蚊的触角也对乙醛有反应。笼内产卵和双重选择试验表明，乙醛是一种浓度范围广泛的产卵引诱剂，因此具有潜在的实际应用价值。
Surveillance of mosquito populations is essential for determining the best timing for intervention as well as for monitoring circulation of new and previously detected pathogens. Trapping gravid females is more effective because they are more likely to be infected than nongravid females. However, better gravid trap attractants are sorely needed to replace fermentation recipes, which are cumbersome, have poor quality control, generate an offensive smell, and do not provide a long-term and consistent source of attractants. By using a reverse chemical ecology approach based on odorant receptors from the southern house mosquito, we have identified that acetaldehyde in a wide range of doses both attracts gravid female mosquitoes and stimulates them to lay eggs in oviposition trays. Pheromones and other semiochemicals play a crucial role in today’s integrated pest and vector management strategies. These semiochemicals are typically discovered by bioassay-guided approaches. Here, we applied a reverse chemical ecology approach; that is, we used olfactory proteins to lead us to putative semiochemicals. Specifically, we used 7 of the top 10 odorant receptors (ORs) most expressed in the antennae of the southern house mosquito, Culex quinquefasciatus, and which are yet to be deorphanized. We expressed these receptors in the Xenopus oocyte recording system and challenged them with a panel of 230 odorants, including physiologically and behaviorally active compounds. Six of the ORs were silent either because they are not functional or a key odorant was missing. CquiOR36, which showed the highest transcript levels of all OR genes in female antennae, was also silent to all odorants in the tested panel, but yielded robust responses when it was accidentally challenged with an old sample of nonanal in ethanol. After confirming that fresh samples were inactive and through a careful investigation of all possible “contaminants” in the old nonanal samples, we identified the active ligand as acetaldehyde. That acetaldehyde is activating CquiOR36 was further confirmed by electroantennogram recordings from antennae of fruit flies engineered to carry CquiOR36. Antennae of female mosquitoes also responded to acetaldehyde. Cage oviposition and dual-choice assays demonstrated that acetaldehyde is an oviposition attractant in a wide range of concentrations and thus of potential practical applications.
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影响因子：
3.7
作者：
Xu P;Garczynski SF;Atungulu E;Syed Z;Choo YM;Vidal DM;Zitelli CH;Leal WS
通讯作者：
Leal WS
影响因子：
3.8
作者：
Zhu F;Xu P;Barbosa RM;Choo YM;Leal WS
通讯作者：
Leal WS
DOI：
10.1073/pnas.1316059110
发表时间：
2013-11-12
影响因子：
11.1
作者：
Leal, Walter S.;Choo, Young-Moo;Ueira-Vieira, Carlos
通讯作者：
Ueira-Vieira, Carlos
影响因子：
1
作者：
Cilek, James E.;Weston, Joshua R.;Richardson, Alec G.
通讯作者：
Richardson, Alec G.
影响因子：
3.5
作者：
Hughes, David T.;Pelletier, Julien;Luetje, Charles W.
通讯作者：
Luetje, Charles W.