Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017-2018 yellow fever outbreaks in Brazil.
Neighbor danger: Yellow fever virus epizootics in urban and urban-rural transition areas of Minas Gerais state, during 2017-2018 yellow fever outbreaks in Brazil.
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DOI：
10.1371/journal.pntd.0008658
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发表时间：
2020-10
影响因子：
3.8
通讯作者：
Drumond BP
中科院分区：
文献类型：
--
作者：
Sacchetto L;Silva NIO;Rezende IM;Arruda MS;Costa TA;de Mello ÉM;Oliveira GFG;Alves PA;de Mendonça VE;Stumpp RGAV;Prado AIA;Paglia AP;Perini FA;Lacerda Nogueira M;Kroon EG;de Thoisy B;Trindade GS;Drumond BP
从2016年底到2019年初，巴西面临着大规模的森林黄热病(YF)疫情。2016-2019年的YF疫情影响了人口稠密的地区，特别是东南部地区，导致数千人和非人类灵长类动物(NHP)死亡。我们于2017年1月至2018年12月在米纳斯吉拉斯州城市、城乡交界处和农村地区收集了781具NHP身体，进行了黄热病病毒(YFV)RNA的分子调查。根据采样周期、NHP属、采样面积和采样面积/NHP属对样本进行分析，以比较YFV阳性身体的比例和估计的YFV基因组载量。在来自城市、城乡交界处和农村地区的NHP身体(包括Alouatta属、Callicebus属、Callithrix属和Sapajus属)中，38.1%的身体被确认感染YFV。YFV RNA检测与流行时段(尤其是12~3月)和农村环境呈正相关。在农村地区收集的身体中，估计病毒基因组载量的中位数比城市高(100万倍)。结果表明，青枯病在米纳斯吉拉斯流行期间和非流行期间均有广泛发生。根据YF的演变模式，观察到病毒从农村向城市传播的梯度。在城市地区收集的NHP身体有很高的YF阳性，在米纳斯吉拉斯的67个城市普遍出现，包括大型城市中心。虽然在2016-2019年巴西没有记录在案的城市/伊蚊YFV传播给人类的病例，但在埃及伊蚊高感染的城市地区感染YFV的NHP对YFV城市/伊蚊传播和城市化构成风险。巴西在2016-2019年面临最大规模的森林黄热病(YF)疫情。疫情影响到人口高度稠密的地区，米纳斯吉拉斯州是受影响最严重的州，有数千人和非人类灵长类动物(NHP)死亡。我们调查了2017年和2018年在米纳斯吉拉斯州采集的NHP身体中的黄热病病毒(YFV)RNA。我们展示了YFV感染NHP的广泛发生，包括2017年非流行旱季的病毒持久性。在城市、城乡交界处和农村地区的NHP身体中检测到YFV RNA。我们还在49个城市发现了新的YF病例，这些城市以前在疫情期间没有发现YF病例。对自然感染的NHP身体中YFV基因组载量的估计显示，在农村地区采集的样本(Alouatta、Callithrix和Callicebus)的基因组载量较高且相似，而在城乡交界处和城市Callithrix样本的基因组载量较低。YFV在城市地区的存在构成迫在眉睫的风险，尽管在巴西最近一次暴发期间，没有人感染病例与城市/伊蚊传播有关。
From the end of 2016 until the beginning of 2019, Brazil faced a massive sylvatic yellow fever (YF) outbreak. The 2016–2019 YF epidemics affected densely populated areas, especially the Southeast region, causing thousands of deaths of humans and non-human primates (NHP). We conducted a molecular investigation of yellow fever virus (YFV) RNA in 781 NHP carcasses collected in the urban, urban-rural interface, and rural areas of Minas Gerais state, from January 2017 to December 2018. Samples were analyzed according to the period of sampling, NHP genera, sampling areas, and sampling areas/NHP genera to compare the proportions of YFV-positive carcasses and the estimated YFV genomic loads. YFV infection was confirmed in 38.1% of NHP carcasses (including specimens of the genera Alouatta, Callicebus, Callithrix, and Sapajus), from the urban, urban-rural interface, and rural areas. YFV RNA detection was positively associated with epidemic periods (especially from December to March) and the rural environment. Higher median viral genomic loads (one million times) were estimated in carcasses collected in rural areas compared to urban ones. The results showed the wide occurrence of YF in Minas Gerais in epidemic and non-epidemic periods. According to the sylvatic pattern of YF, a gradient of viral dissemination from rural towards urban areas was observed. A high YF positivity was observed for NHP carcasses collected in urban areas with a widespread occurrence in 67 municipalities of Minas Gerais, including large urban centers. Although there was no documented case of urban/Aedes YFV transmission to humans in Brazil during the 2016–2019 outbreaks, YFV-infected NHP in urban areas with high infestation by Aedes aegypti poses risks for YFV urban/Aedes transmission and urbanization. Brazil faced the most massive sylvatic yellow fever (YF) outbreak in 2016–2019. The outbreak affected highly densely populated areas, and Minas Gerais was the most affected state with thousands of deaths of human and non-human primates (NHP). We investigated the yellow fever virus (YFV) RNA in NHP carcasses collected throughout Minas Gerais in 2017 and 2018. We demonstrated the wide occurrence of YFV-infected NHP, including the viral persistence during the non-epidemic dry season of 2017. YFV RNA was detected in NHP carcasses in the urban, urban-rural interface and rural areas. We have also detected new YF cases in 49 municipalities where YF cases have not been previously detected during the outbreaks. Estimates of YFV genomic load in naturally infected NHP carcasses showed high and similar loads in specimens (Alouatta, Callithrix, and Callicebus) collected in rural areas and lower genomic loads in the urban-rural interface and urban Callithrix specimens. The presence of YFV inside urban areas poses an imminent risk, although no human case was epidemiologically linked to urban/Aedes transmission during the last outbreaks in Brazil.
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DOI：
10.1590/s0101-81752006000100005
发表时间：
2006-03-01
期刊：
Revista Brasileira de Zoologia
影响因子：
--
作者：
Gregorin, Renato
通讯作者：
Gregorin, Renato
影响因子：
3.8
作者：
Gomez, Mariela Martinez;Santos de Abreu, Filipe Vieira;Bonaldo, Myrna Cristina
通讯作者：
Bonaldo, Myrna Cristina
DOI：
10.1590/s0034-89102010005000046
发表时间：
2010-12-01
期刊：
Revista de Saúde Pública
影响因子：
--
作者：
Vasconcelos, Pedro Fernando da Costa
通讯作者：
Vasconcelos, Pedro Fernando da Costa
影响因子：
4.6
作者：
Mir, Daiana;Delatorre, Edson;Bello, Gonzalo
通讯作者：
Bello, Gonzalo
影响因子：
3.3
作者：
CLARK, HC
通讯作者：
CLARK, HC