Duplication and Specialization of NUDX1 in Rosaceae Led to Geraniol Production in Rose Petals.
Duplication and Specialization of NUDX1 in Rosaceae Led to Geraniol Production in Rose Petals.
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DOI：
10.1093/molbev/msac002
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发表时间：
2022-02-03
影响因子：
10.7
通讯作者：
Caissard JC
中科院分区：
文献类型：
--
作者：
Conart C;Saclier N;Foucher F;Goubert C;Rius-Bony A;Paramita SN;Moja S;Thouroude T;Douady C;Sun P;Nairaud B;Saint-Marcoux D;Bahut M;Jeauffre J;Hibrand Saint-Oyant L;Schuurink RC;Magnard JL;Boachon B;Dudareva N;Baudino S;Caissard JC
营养素水解酶是普遍存在于所有生命王国中的保守酶。近年来的研究表明，植物中有几种营养素水解酶参与萜类化合物的代谢。在现代玫瑰中，RhNUDX 1负责形成香叶醇，这是玫瑰香味的主要化合物。然而，这种化合物是由许多生产香叶醇的植物中的单萜脱氢酶产生的。因此，这提出了关于RhNUDX 1功能的起源和NUDX 1基因在蔷薇科、野生玫瑰或/和驯化过程中的进化的问题。在这里，我们发现，三个不同的分支NUDX 1出现在蔷薇科亚科（Nudx 1 -1至Nudx 1 -3分支），和两个亚分支进化在蔷薇属（Nudx 1 -1a和Nudx 1 -1b亚分支）。我们还表明，Nudx 1 -1b亚支比Nudx 1 -1a亚支更古老，NUDX 1 -1a基因是由更古老的NUDX 1 -1b基因的反式复制产生的。转座后，NUDX 1 -1a顺式复制，导致不同物种中香叶醇产量的基因剂量效应。此外，NUDX 1 -1a的出现伴随着其启动子的进化，最有可能来自Copia反转录转座子来源，导致其花瓣特异性表达。因此，我们的数据有力地表明，NUDX 1 -1a在香叶醇形成中的独特功能是在驯化之前在蔷薇属中自然进化的。
Nudix hydrolases are conserved enzymes ubiquitously present in all kingdoms of life. Recent research revealed that several Nudix hydrolases are involved in terpenoid metabolism in plants. In modern roses, RhNUDX1 is responsible for formation of geraniol, a major compound of rose scent. Nevertheless, this compound is produced by monoterpene synthases in many geraniol-producing plants. As a consequence, this raised the question about the origin of RhNUDX1 function and the NUDX1 gene evolution in Rosaceae, in wild roses or/and during the domestication process. Here, we showed that three distinct clades of NUDX1 emerged in the Rosoidae subfamily (Nudx1-1 to Nudx1-3 clades), and two subclades evolved in the Rosa genus (Nudx1-1a and Nudx1-1b subclades). We also showed that the Nudx1-1b subclade was more ancient than the Nudx1-1a subclade, and that the NUDX1-1a gene emerged by a trans-duplication of the more ancient NUDX1-1b gene. After the transposition, NUDX1-1a was cis-duplicated, leading to a gene dosage effect on the production of geraniol in different species. Furthermore, the NUDX1-1a appearance was accompanied by the evolution of its promoter, most likely from a Copia retrotransposon origin, leading to its petal-specific expression. Thus, our data strongly suggest that the unique function of NUDX1-1a in geraniol formation was evolved naturally in the genus Rosa before domestication.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
9.2
作者：
Buti M;Moretto M;Barghini E;Mascagni F;Natali L;Brilli M;Lomsadze A;Sonego P;Giongo L;Alonge M;Velasco R;Varotto C;Šurbanovski N;Borodovsky M;Ward JA;Engelen K;Cavallini A;Cestaro A;Sargent DJ
通讯作者：
Sargent DJ
草莓中组蛋白赖氨酸甲基化修饰剂的鉴定和表征（正在出版。DOI：10.1038/srep23581）
影响因子：
4.6
作者：
Gu T;Han Y;Huang R;McAvoy RJ;Li Y
通讯作者：
Li Y
影响因子：
4.4
作者：
Dubois A;Carrere S;Raymond O;Pouvreau B;Cottret L;Roccia A;Onesto JP;Sakr S;Atanassova R;Baudino S;Foucher F;Le Bris M;Gouzy J;Bendahmane M
通讯作者：
Bendahmane M
DOI：
10.1016/j.bbagrm.2014.07.017
发表时间：
2015-04-01
影响因子：
4.7
作者：
Grandbastien, Marie-Angele
通讯作者：
Grandbastien, Marie-Angele