Distinct cellular pools of perilipin 5 point to roles in lipid trafficking.
Distinct cellular pools of perilipin 5 point to roles in lipid trafficking.
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DOI：
10.1016/j.bbalip.2011.10.017
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Tansey JT
中科院分区：
文献类型：
--
作者：
Bartholomew SR;Bell EH;Summerfield T;Newman LC;Miller EL;Patterson B;Niday ZP;Ackerman WE 4th;Tansey JT
脂质储存滴蛋白PAT家族由五个成员组成，每个成员都已成为细胞中性脂质代谢的既定调节因子。Perilipin 5（也被称为lsdp-5， MLDP， PAT-1和OXPAT）是最近发现的家族成员，已被证明定位于两个不同的细胞内池：脂质储存液滴（LD）和一个特征不明确的细胞质部分。我们已经描述了这些细胞内池的密度，并发现与大ld无关的perilipin 5种群存在于离散密度（~ 1.15 g/ml）和大小（~ 575 kDa）的复合物中。通过免疫荧光、分离蔗糖密度组分的western blotting、天然梯度凝胶电泳和共免疫沉淀，我们已经证明这些小的（~ 15 nm）、perilipin 5包被的结构不包含PAT蛋白perilipin 2 (ADRP)，但确实包含perilipin 3和其他一些尚未表征的蛋白质。这些颗粒的大小和密度以及它们对脂肪酶降解的敏感性表明，像较大的ld一样，它们具有中性的富含脂质的核心。当油酸处理促进中性脂质沉积时，异位表达periilipin 5的细胞经历了细胞内ld的重组，导致小液滴减少，大液滴减少。总的来说，这些数据表明，一部分细胞质内的外壁素5存在于参与细胞中性脂质积累的高密度脂滴复合物中。
The PAT family of lipid storage droplet proteins comprised five members, each of which has become an established regulator of cellular neutral lipid metabolism. Perilipin 5 (also known as lsdp-5, MLDP, PAT-1, and OXPAT), the most recently discovered member of the family, has been shown to localize to two distinct intracellular pools: the lipid storage droplet (LD), and a poorly characterized cytosolic fraction. We have characterized the denser of these intracellular pools and find that a population of perilipin 5 not associated with large LDs resides in complexes with a discrete density (~ 1.15 g/ml) and size (~ 575 kDa). Using immunofluorescence, western blotting of isolated sucrose density fractions, native gradient gel electrophoresis, and co-immunoprecipitation, we have shown that these small (~ 15 nm), perilipin 5-encoated structures do not contain the PAT protein perilipin 2 (ADRP), but do contain perilipin 3 and several other as of yet uncharacterized proteins. The size and density of these particles as well as their susceptibility to degradation by lipases suggest that like larger LDs, they have a neutral lipid rich core. When treated with oleic acid to promote neutral lipid deposition, cells ectopically expressing perilipin 5 experienced a reorganization of LDs in the cell, resulting in fewer, larger droplets at the expense of smaller ones. Collectively, these data demonstrate that a portion of cytosolic perilipin 5 resides in high density lipid droplet complexes that participate in cellular neutral lipid accumulation.
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DOI：
10.1083/jcb.200510010
发表时间：
2006-06-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
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发表时间：
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影响因子：
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