Enzyme II of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: protein-protein and protein-phospholipid interactions.
Enzyme II of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: protein-protein and protein-phospholipid interactions.
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大肠杆菌磷酸烯醇丙酮酸依赖性磷酸转移酶系统的酶 II：蛋白质-蛋白质和蛋白质-磷脂相互作用。
作者：
G. Robillard;Mieke Blaauw
甘露醇特异性酶II（EII），纯化的磷脂，表现出浓度依赖性的比活性与P-HPr和甘露醇作为供体和受体底物，分别。以前仅在甘露醇-甘露醇磷酸交换反应中观察到的这种浓度依赖性表明，酶的低聚物形式负责催化磷酸化反应（P-HPr +甘露醇-甘露醇-P + HPr）以及交换反应。动力学分析表明，单体酶的比活性比相关物种低得多。通过提高EII磷酸化的稳态水平以及添加磷脂，可以增加比活性，这表明磷酸化和磷脂的结合促进了缔合过程。动力学测量和荧光能量转移测量表明，强烈的偏好EII磷脂与特定的头基和脂肪酸组成。
The mannitol-specific enzyme II (EII), purified free of phospholipid, exhibits a concentration dependence in its specific activity with P-HPr and mannitol as the donor and acceptor substrates, respectively. This concentration dependence, previously observed only in the case of mannitol----mannitol phosphate exchange reaction, indicates that an oligomeric form of the enzyme is responsible for catalyzing the phosphorylation reaction (P-HPr + mannitol----mannitol-P + HPr) as well as the exchange reaction. Kinetic analysis revealed that the monomeric enzyme has a much lower specific activity than the associated species. The specific activity can be increased by raising the steady-state level of phosphorylation of EII and also by adding phospholipid, demonstrating that phosphorylation and the binding of phospholipid facilitate the association process. Kinetic measurements and fluorescence energy transfer measurements demonstrate a strong preference of EII for phospholipids with specific head group and fatty acid composition.
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DOI：
10.1016/s0021-9258(17)44521-2
发表时间：
1983-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. E. Leonard;M. Saier
通讯作者：
J. E. Leonard;M. Saier
大肠杆菌甘露醇渗透酶的亚基相互作用：与酶活性的相关性。
影响因子：
2.9
作者：
Stephan,MM;Jacobson,GR
通讯作者：
Jacobson,GR
细菌磷酸转移酶系统的甘露醇特异性酶 II。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jacobson,GR;Lee,CA;Leonard,JE;SaierJr,MH
通讯作者：
SaierJr,MH
与细菌磷酸转移酶系统的酶 II 相关的催化活性。
DOI：
10.1002/jss.400140303
发表时间：
1980
期刊：
Journal of supramolecular structure
影响因子：
--
作者：
SaierJr,MH
通讯作者：
SaierJr,MH