Herpes simplex virus type-1 (HSV-1) entry into human mesenchymal stem cells is heavily dependent on heparan sulfate.
Herpes simplex virus type-1 (HSV-1) entry into human mesenchymal stem cells is heavily dependent on heparan sulfate.
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DOI：
10.1155/2011/264350
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发表时间：
2011
影响因子：
--
通讯作者：
Tiwari V
中科院分区：
文献类型：
--
作者：
Choudhary S;Marquez M;Alencastro F;Spors F;Zhao Y;Tiwari V
造血干细胞接受者仍然容易受到包括1型单纯疱疹病毒(HSV-1)在内的机会性病毒感染。本研究的目的是分析人骨髓间充质干细胞(HMSCs)对HSV-1感染的易感性，并确定主要的进入受体。合胞型HSV-1 Kos(804)病毒复制和空斑形成试验证实hMSCs中存在病毒感染。为了检测进入受体的意义，进行了RT-PCR和抗体阻断试验。RT-PCR结果显示GD受体：Nectin-1，3-O-SulfoTransfer-3(3-OST-3)和Hvem的表达。抗体阻断试验和肝素酶处理表明，HS和3-O-硫酸肝素(3-OS HS)在介导HSV-1进入和在hMSCs中传播中起重要作用，而Nectin-1或Hvem不起作用。综上所述，我们的结果提供了强有力的证据，表明HSV-1能够感染hMSCs，HS和3-OS HS在这一过程中起到进入受体的作用。
Hematopoietic stem cells recipients remain susceptible to opportunistic viral infections including herpes simplex virus type-1 (HSV-1). The purpose of this investigation was to analyze susceptibility of human mesenchymal stem cells (hMSCs) to HSV-1 infection and identify the major entry receptor. Productive virus infection in hMSCs was confirmed by replication and plaque formation assays using a syncytial HSV-1 KOS (804) virus. To examine the significance of entry receptors, RT-PCR and antibody-blocking assays were performed. RT-PCR data showed the expression of gD receptors: nectin-1, 3-O sulfotransferase-3 (3-OST-3), and HVEM. Antibody-blocking assay together with heparinase treatment suggested an important role for HS and 3-O-sulfated heparan sulfate (3-OS HS), but not nectin-1 or HVEM, in mediating HSV-1 entry and spread in hMSCs. Taken together, our results provide strong evidence demonstrating that HSV-1 is capable of infecting hMSCs and HS and 3-OS HS serve as its entry receptors during this process.
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