Rapid generation of rice mutants via the dominant negative suppression of the mismatch repair protein OsPMS1
Rapid generation of rice mutants via the dominant negative suppression of the mismatch repair protein OsPMS1
复制标题
通过错配修复蛋白 OsPMS1 的显性失活抑制快速产生水稻突变体
DOI：
10.1007/s00122-012-1888-5
复制
发表时间：
2012-06
影响因子：
5.4
通讯作者：
Li, Hongqing
中科院分区：
文献类型：
--
作者：
Li, Meiru;Chen, Lei;Wu, Guojiang;Li, Hongqing
错配修复（MMR）是维持不同生物体基因组完整性的保守途径。尽管MMR抑制在拟南芥中导致了各种突变表型，但在主要作物中使用这种策略进行突变育种还没有报道。在这里，我们在水稻中过表达OsPMS1蛋白的截短版本;这种方法有望通过显性负性机制抑制水稻MMR系统。在转基因植株的种子萌发和植株生长过程中，我们观察到了广泛的突变表型。用简单重复序列（inter-simple sequence repeat，ISSR）方法检测基因组变异，并对差异条带进行测序，结果表明该突变以点突变或微卫星不稳定性的形式发生。获得了具有重要农艺性状的株系，如作为盐和耐旱性、各种分蘖数和早花。此外，我们获得了具有重要性状的突变体，这些突变体不含转基因。总之，这些结果表明，MMR抑制可以用作水稻突变育种的有效策略。
Mismatch repair (MMR) is a conservative pathway for maintaining the genome integrity of different organisms. Although suppression of MMR has resulted in various mutation phenotypes inArabidopsis, the use of this strategy for mutation breeding in major crops has not been reported. Here, we overexpressed a truncated version of the OsPMS1 protein in rice; this approach is expected to suppress the rice MMR system through a dominant negative mechanism. We observed a wide spectrum of mutation phenotypes in the progeny of the transgenic plants during seed germination and the plant growth stages. Genomic variations were detected with inter-simple sequence repeat (ISSR), and sequencing of the differential ISSR bands revealed that the mutation occurred as a point mutation or as microsatellite instability at high frequencies. Plant lines with agronomically important traits, such as salt and drought tolerance, various tiller number, and early flowering, were obtained. Furthermore, we obtained mutants with important traits that are free of the transgene. Together, these results demonstrate that MMR suppression can be used as an efficient strategy for mutation breeding in rice.
登录
查看更多内容
影响因子：
2.7
作者：
H. Chen;R. S. Nelson;Sherwood Jl
通讯作者：
H. Chen;R. S. Nelson;Sherwood Jl
DOI：
--
发表时间：
2003-08
期刊：
Shi yan sheng wu xue bao
影响因子：
--
作者：
Mei-Ru Li;Hong Li
通讯作者：
Mei-Ru Li;Hong Li
影响因子：
64.8
作者：
BRONNER, CE;BAKER, SM;LISKAY, RM
通讯作者：
LISKAY, RM
DOI：
--
发表时间：
2001
期刊：
--
影响因子：
--
作者：
LongPing Yuan;S. Peng;B. Hardy
通讯作者：
LongPing Yuan;S. Peng;B. Hardy
DOI：
--
发表时间：
1999
期刊：
--
影响因子：
--
作者：
C. W. Smith;J. T. Cothren
通讯作者：
C. W. Smith;J. T. Cothren