Current methods for diagnosis of human coronaviruses: pros and cons.
Current methods for diagnosis of human coronaviruses: pros and cons.
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当前诊断人冠状病毒的方法：利弊。
DOI：
10.1007/s00216-020-03046-0
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发表时间：
2021-04
影响因子：
4.3
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Benzigar MR;Bhattacharjee R;Baharfar M;Liu G
世界卫生组织（WHO）认为，目前还没有能够立即诊断或治愈新冠肺炎的方法，因此提出了“为使传播曲线扁平化而展开的全球防疫战”。为了阻止传播，世界各地的研究人员正在夜以继日地工作，旨在开发可靠的工具，用于早期诊断严重急性呼吸系统综合征（SARS-CoV-2），了解感染途径和机制。目前，基于核酸的分子诊断（实时逆转录聚合酶链反应（RT-PCR）检测）被认为是早期诊断SARS-CoV-2的金标准。基于抗体的血清学检测对于早期诊断是无效的，但它是一种潜在的血清调查工具，可以为人们提供清除COVID-19感染的免疫证明。同时，开花方法也多种多样。在这篇综述中，我们总结了发现的不同类型的冠状病毒，它们可以在人与人之间传播。介绍了新发现的人类冠状病毒（SARS、MERS、COVID-19）的核酸检测、基因测序、抗体检测、抗原检测和临床诊断等诊断方法。讨论了它们的优缺点和前景，有助于研究人员开发新一代先进的诊断工具，以准确有效地控制人类冠状病毒在社区和医院的传播。
The current global fight against coronavirus disease (COVID-19) to flatten the transmission curve is put forth by the World Health Organization (WHO) as there is no immediate diagnosis or cure for COVID-19 so far. In order to stop the spread, researchers worldwide are working around the clock aiming to develop reliable tools for early diagnosis of severe acute respiratory syndrome (SARS-CoV-2) understanding the infection path and mechanisms. Currently, nucleic acid-based molecular diagnosis (real-time reverse transcription polymerase chain reaction (RT-PCR) test) is considered the gold standard for early diagnosis of SARS-CoV-2. Antibody-based serology detection is ineffective for the purpose of early diagnosis, but a potential tool for serosurveys, providing people with immune certificates for clearance from COVID-19 infection. Meanwhile, there are various blooming methods developed these days. In this review, we summarise different types of coronavirus discovered which can be transmitted between human beings. Methods used for diagnosis of the discovered human coronavirus (SARS, MERS, COVID-19) including nucleic acid detection, gene sequencing, antibody detection, antigen detection, and clinical diagnosis are presented. Their merits, demerits and prospects are discussed which can help the researchers to develop new generation of advanced diagnostic tools for accurate and effective control of human coronavirus transmission in the communities and hospitals.
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影响因子：
4.6
作者：
Chamings A;Nelson TM;Vibin J;Wille M;Klaassen M;Alexandersen S
通讯作者：
Alexandersen S
DOI：
10.1126/science.aaf5573
发表时间：
2016-08-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Abudayyeh OO;Gootenberg JS;Konermann S;Joung J;Slaymaker IM;Cox DB;Shmakov S;Makarova KS;Semenova E;Minakhin L;Severinov K;Regev A;Lander ES;Koonin EV;Zhang F
通讯作者：
Zhang F
DOI：
10.1073/pnas.1919800117
发表时间：
2020-02-11
影响因子：
11.1
作者：
Di, Lin;Fu, Yusi;Wang, Jianbin
通讯作者：
Wang, Jianbin
影响因子：
9.3
作者：
Behrmann, Ole;Bachmann, Iris;Hufert, Frank T.
通讯作者：
Hufert, Frank T.
影响因子：
7.6
作者：
Chang CK;Hou MH;Chang CF;Hsiao CD;Huang TH
通讯作者：
Huang TH