Sequential injection reductive bio-sorption of Cr(VI) on the surface of egg-shell membrane and chromium speciation with detection by electrothermal atomic absorption spectrometry
Sequential injection reductive bio-sorption of Cr(VI) on the surface of egg-shell membrane and chromium speciation with detection by electrothermal atomic absorption spectrometry
复制标题
蛋壳膜表面序贯注射还原生物吸附 Cr(VI) 及铬形态并用电热原子吸收光谱法检测
DOI：
10.1039/b714535g
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发表时间：
2008-02
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
--
作者：
卵壳膜（ESM）是一种具有多种功能基团的独特细胞表面，为金属物质的生物吸附提供了潜力。我们已经证明了铬（VI）的选择性保留通过还原吸附过程，而铬（III）实际上不保留。这包括Cr（VI）在ESM表面的吸附，而ESM上的电荷转移导致Cr（VI）通过瞬态、不稳定的Cr(V)和Cr（IV）物质还原为Cr（III）。因此，采用电热原子吸收光谱法在顺序注射系统中开发了一种新的铬形态形成方法。过程包括在pH为2的ESM上对Cr（VI）进行分离和预富集，洗脱后进行检测，然后将Cr（III）转化为Cr（VI）并进行总铬分析，最后通过减法得到Cr（III）。样品体积为1000µl时，富集系数为13.3。Cr（VI）的线性范围为0.05 ~ 1.25µg l−1，检出限为0.01µg l−1，在0.5µg l−1的水平上精度为3.2%。采用经认证的河水（GBW08608）和洞穴水标准物质进行铬形态分析。
Egg-shell membrane (ESM) is a unique cell surface with various functional groups, providing the potential for bio-sorption of metal species. We have demonstrated the selective retention of chromium(VI)via a reductive sorption process, while Cr(III) is virtually not retained. This includes adsorption of Cr(VI) onto the ESM surface while charge transfer on the ESM results in the reduction of Cr(VI) to Cr(III) through transient, unstable species of Cr(V) and Cr(IV). A novel procedure for chromium speciation was thus developed in a sequential injection system with detection by electrothermal atomic absorption spectrometry. The procedure includes the separation and preconcentration of Cr(VI) on ESM at pH 2 and its subsequent detection after elution, followed by conversion of Cr(III) to Cr(VI) and total chromium analysis, Cr(III) finally being obtained by subtraction. With a sampling volume of 1000 µl, an enrichment factor of 13.3 was achieved. A linear range of 0.05–1.25 µg l−1 for Cr(VI), along with a detection limit of 0.01 µg l−1 and a precision of 3.2% at the level of 0.5 µg l−1, were obtained. Chromium speciation was performed by using a certified reference material of riverine water (GBW08608) and cave water.
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影响因子：
3.9
作者：
S. Ishikawa;K. Suyama;K. Arihara;M. Itoh
通讯作者：
S. Ishikawa;K. Suyama;K. Arihara;M. Itoh
影响因子：
6.1
作者：
Baycin, Deniz;Altiok, Evren;Bayraktar, Oguz
通讯作者：
Bayraktar, Oguz
影响因子：
--
作者：
Dong Yang;L. Qi;Jiming Ma
通讯作者：
Dong Yang;L. Qi;Jiming Ma
影响因子：
4.4
作者：
PARSONS, AH
通讯作者：
PARSONS, AH
影响因子：
6.2
作者：
M. El-Shahawi;S. Hassan;A. Othman;M. A. Zyada;M. A. El-Sonbati
通讯作者：
M. El-Shahawi;S. Hassan;A. Othman;M. A. Zyada;M. A. El-Sonbati