LC/MS-based quantitative proteomic analysis of paraffin-embedded archival melanomas reveals potential proteomic biomarkers associated with metastasis.
LC/MS-based quantitative proteomic analysis of paraffin-embedded archival melanomas reveals potential proteomic biomarkers associated with metastasis.
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DOI：
10.1371/journal.pone.0004430
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Hoon DS
中科院分区：
文献类型：
--
作者：
Huang SK;Darfler MM;Nicholl MB;You J;Bemis KG;Tegeler TJ;Wang M;Wery JP;Chong KK;Nguyen L;Scolyer RA;Hoon DS
黑色素瘤转移状态与患者的整体生存率高度相关。然而，对于黑色素瘤肿瘤进展过程中蛋白质组学的变化知之甚少。为了更好地了解黑色素瘤进展和转移涉及的蛋白质表达的变化，并确定潜在的生物标志物，我们对档案转移和原发性黑色素瘤进行了全球定量蛋白质组学分析。
总共评估了16个转移性和8个原发性皮肤黑色素瘤。通过液化组织细胞从激光捕获的福尔马林固定石蜡包裹的档案组织中提取蛋白质。通过基于LC/MS的无标签蛋白定量方法分析这些制剂。在肽ID置信度> 75％的组织裂解物中发现了1500多种蛋白质。这种方法确定了蛋白质水平的120个重大变化。这些蛋白质是从具有高置信度鉴定的多种肽中鉴定出来的，与原代黑色素瘤相比，在转移酶的水平显着差异（Q值<0.05）。
通过生物过程将差异表达的蛋白质分类或映射到生物系统网络中，这些分析与癌症或转移相关的几种蛋白质与几种蛋白质相关。这些蛋白质代表了肿瘤进展的潜在生物标志物。该研究成功地鉴定出在福尔马林固定石蜡包裹的转移性和原发性黑色素瘤标本中差异表达的蛋白质。
Melanoma metastasis status is highly associated with the overall survival of patients; yet, little is known about proteomic changes during melanoma tumor progression. To better understand the changes in protein expression involved in melanoma progression and metastasis, and to identify potential biomarkers, we conducted a global quantitative proteomic analysis on archival metastatic and primary melanomas. A total of 16 metastatic and 8 primary cutaneous melanomas were assessed. Proteins were extracted from laser captured microdissected formalin fixed paraffin-embedded archival tissues by liquefying tissue cells. These preparations were analyzed by a LC/MS-based label-free protein quantification method. More than 1500 proteins were identified in the tissue lysates with a peptide ID confidence level of >75%. This approach identified 120 significant changes in protein levels. These proteins were identified from multiple peptides with high confidence identification and were expressed at significantly different levels in metastases as compared with primary melanomas (q-Value<0.05). The differentially expressed proteins were classified by biological process or mapped into biological system networks, and several proteins were implicated by these analyses as cancer- or metastasis-related. These proteins represent potential biomarkers for tumor progression. The study successfully identified proteins that are differentially expressed in formalin fixed paraffin-embedded specimens of metastatic and primary melanoma.
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DOI：
10.1002/0471728780.ch4
发表时间：
2005-01-01
期刊：
DRUG DISCOVERY HANDBOOK
影响因子：
--
作者：
Ekins, Sean;Bugrim, Andrej;Nikolskaya, Tatiana
通讯作者：
Nikolskaya, Tatiana
影响因子：
7
作者：
Hood, BL;Darfler, MM;Krizman, DB
通讯作者：
Krizman, DB
影响因子：
6.2
作者：
Kunkel, M;Reichert, TE;Whiteside, TL
通讯作者：
Whiteside, TL
影响因子：
50.3
作者：
Kim, RH;Peters, M;Mak, TW
通讯作者：
Mak, TW
影响因子：
5.8
作者：
Craig, R;Beavis, RC
通讯作者：
Beavis, RC