Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro.
Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro.
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DOI：
10.1016/j.phytochem.2014.03.028
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发表时间：
2014-07
期刊：
影响因子：
3.8
通讯作者：
Raskin I
中科院分区：
文献类型：
--
作者：
Waterman C;Cheng DM;Rojas-Silva P;Poulev A;Dreifus J;Lila MA;Raskin I
辣木（辣木）是一种可食用的植物，在整个热带地区用作食物和药物。一种通过用水提取新鲜叶子制备的辣木浓缩物（MC），利用天然存在的黑芥子酶将四种辣木芥子油苷（1-4）转化为辣木异硫氰酸酯（5-8）。确定了使MC产率、化合物5（4-[（α-L-鼠李糖氧基）苄基]异硫氰酸酯）和化合物8（4-[（4 '-O-乙酰基-α-L-鼠李糖氧基）苄基]异硫氰酸酯）含量最大化的最佳条件（室温下鲜叶重量与水的比例为1：5）。优化后的MC中异硫氰酸酯含量为1.66%，总多酚含量为3.82%。化合物8在37 °C下30天表现出80%的稳定性。MC、5和8显著降低RAW巨噬细胞中炎症标志物的基因表达和产生。具体而言，5和8在1和5 μM时减弱了iNOS和IL-1β的表达以及一氧化氮和TNFβ的产生。我们的研究结果表明，作为食品级产品在MC中递送的稳定和浓缩的辣木异硫氰酸酯（5-8）有可能减轻与慢性疾病相关的低度炎症。
Moringa (Moringa oleifera Lam.) is an edible plant used as food and medicine throughout the tropics. A moringa concentrate (MC) made by extracting fresh leaves with water utilized naturally occurring myrosinase to convert four moringa glucosinolates (1–4) into moringa isothiocyanates (5–8). Optimum conditions maximizing MC yield, compound 5 (4-[(α-L-rhamnosyloxy)benzyl]isothiocyanate), and compound 8 (4-[(4’-O-acetyl-α-L-rhamnosyloxy)benzyl]isothiocyanate) content were established (1:5 fresh leaf weight to water ratio at room temperature). The optimized MC contained 1.66% isothiocyanates and 3.82% total polyphenols. Compound 8 exhibited 80% stability at 37 °C for 30 days. MC, 5, and 8 significantly decreased gene expression and production of inflammatory markers in RAW macrophages. Specifically, 5 and 8 attenuated expression of iNOS and IL-1β and production of nitric oxide and TNFβ at 1 and 5 µM. Our results suggest a potential for stable and concentrated moringa isothiocyanates (5–8), delivered in MC as a food-grade product, to alleviate low-grade inflammation associated with chronic diseases.
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