Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage phi29 DNA packaging motor.
Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage phi29 DNA packaging motor.
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DOI：
10.1021/nn900187k
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发表时间：
2009-08-25
期刊：
影响因子：
17.1
通讯作者：
Guo, Peixuan
中科院分区：
文献类型：
--
作者：
Xiao, Feng;Cai, Ying;Wang, Joseph Che-Yen;Green, Dominik;Cheng, R. Holland;Demeler, Borries;Guo, Peixuan
关键词：
构建了一个24 × 30 nm的椭圆形纳米颗粒，其中包含84个亚基或7个十二聚体的噬菌体phi 29 DNA包装马达的重组核心蛋白。通过简单的一步纯化得到均匀的纳米颗粒。电子显微镜和分析ultracenthegation阐明的结构，形状，尺寸和组装机制。这种结构的形成是由N-末端肽延伸介导和稳定的。通过用蛋白酶切割延伸的N-末端肽来控制84-亚基椭圆体纳米颗粒对其十二聚体亚基的回复。将84个外向C-末端与可用于掺入标记物的链霉亲和素结合肽缀合。这进一步扩展了这种纳米颗粒通过信号增强进行病原体检测和疾病诊断的应用。
A 24 × 30 nm ellipsoid nanoparticle containing 84 subunits or 7 dodecamers of the re-engineered core protein of the bacteriophage phi29 DNA packaging motor was constructed. Homogeneous nanoparticles were obtained with simple one-step purification. Electron microscopy and analytical ultracentrifugation were employed to elucidate the structure, shape, size, and mechanism of assembly. The formation of this structure was mediated and stabilized by N-terminal peptide extensions. Reversal of the 84-subunit ellipsoid nanoparticle to its dodecamer subunit was controlled by the cleavage of the extended N-terminal peptide with a protease. The 84 outward-oriented C-termini were conjugated with a streptavidin binding peptide which can be used for the incorporation of markers. This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement.
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影响因子：
56.9
作者：
Chworos, A;Severcan, I;Jaeger, L
通讯作者：
Jaeger, L
影响因子：
14.8
作者：
Schmidt, Thomas G. M.;Skerra, Arne
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影响因子：
15
作者：
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通讯作者：
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DOI：
10.1073/pnas.232299399
发表时间：
2002-11-12
影响因子：
11.1
作者：
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通讯作者：
Sára, M
影响因子：
2.4
作者：
Brookes, Emre;Demeler, Borries
通讯作者：
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