Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2.
Heparin-dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2.
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DOI：
10.1002/term.2681
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发表时间：
2018-06
影响因子：
3.3
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Yao Q;Liu Y;Sun H
最近开发的三维（3D）石墨烯泡沫（GrF）对于潜在的骨组织工程应用是有趣的，因为它为干细胞提供了用于成骨分化的3D多孔基底。然而，石墨烯结构的性质缺乏官能团，因此难以进一步修饰，例如生长因子的固定或缀合，这通常是促进组织再生所需的。为了探索GrF功能化和持续释放治疗性蛋白质的潜力，我们使用高度可扩展的化学气相沉积（CVD）方法制造了具有生物启发的肝素-多巴胺（Hepa-Dopa）分子的改性3D GrF支架。我们的数据表明，与未处理的支架相比，Hepa-Dopa修饰导致显著更高的骨形态发生蛋白-2（BMP-2）结合能力和更长的释放能力。重要的是，肝素功能化的3D GrF显著改善了外源性BMP 2诱导的成骨分化。因此，我们的研究首次表明，3D GrF可以用Hepa-Dopa生物模拟功能化，并用于持续释放BMP 2，从而诱导成骨分化，并暗示有希望成为骨组织工程中治疗性蛋白质和干细胞的新的多功能载体。
The recently developed three-dimensional (3D) graphene foam (GrF) is intriguing for potential bone tissue engineering applications since it provides stem cells with a 3D porous substrate for osteogenic differentiation. However, the nature of graphene’s structure lacks functional groups thus making it difficult for further modification such as immobilization or conjugation of growth factors, which are normally required to promote tissue regeneration. To explore the potential of GrF functionalization and sustained release of therapeutic proteins, we fabricated a modified 3D GrF scaffold with bio-inspired heparin-dopamine (Hepa-Dopa) molecules using a highly scalable chemical vapor deposition (CVD) method. Our data indicated Hepa-Dopa modification resulted in significantly higher bone morphogenetic protein-2 (BMP2) binding ability and longer release capacity compared to the untreated scaffolds. Importantly, the heparin-functionalized 3D GrF significantly improved the exogenous BMP2-induced osteogenic differentiation. Therefore, our study, for the first time, indicated that the 3D GrF can be bio-mimetically functionalized with Hepa-Dopa and be used for sustained release of BMP2, thereby inducing osteogenic differentiation and suggesting promising potential as a new multifunctional carrier for therapeutic proteins and stem cells in bone tissue engineering.
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DOI：
10.1016/s0006-291x(03)00858-1
发表时间：
2003-06-13
影响因子：
3.1
作者：
Lu, HH;Kofron, MD;Laurencin, CT
通讯作者：
Laurencin, CT
影响因子：
14
作者：
Kim, Sung Eun;Song, Sang-Hun;Kwon, Yong-Dae
通讯作者：
Kwon, Yong-Dae
影响因子：
56.9
作者：
Lee, Haeshin;Dellatore, Shara M.;Messersmith, Phillip B.
通讯作者：
Messersmith, Phillip B.
影响因子：
2.8
作者：
Hong, J;Cabe, GD;Snyder, BD
通讯作者：
Snyder, BD
影响因子：
5.6
作者：
Kanzaki, Shin;Takahashi, Tetsu;Nishihara, Tatsuji
通讯作者：
Nishihara, Tatsuji