Higher Expression of Collagen-binding Small Leucine-rich Proteoglycan Genes in Mouse Mandibular Bone Marrow than Femoral Bone Marrow
Higher Expression of Collagen-binding Small Leucine-rich Proteoglycan Genes in Mouse Mandibular Bone Marrow than Femoral Bone Marrow
复制标题
小鼠下颌骨髓中富含胶原蛋白结合的小亮氨酸蛋白多糖基因的表达高于股骨骨髓
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
M. Matsuura
中科院分区：
文献类型：
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作者：
Yoshiyuki Nagashima;T. Matsuura;Katsuki Yamamoto;Hironobu Sato;M. Matsuura
虽然颅面骨和上颌骨在胚胎学上从不同的起源发育，并显示出不同的结构和功能，但这些骨中是否存在位点特异性遗传和表型差异仍不清楚。本研究的目的是分析小鼠下颌骨和股骨骨髓中胶原结合小亮氨酸蛋白聚糖（SLRP）和I型胶原（COLI）的基因表达水平，这些蛋白是骨中的主要基质蛋白。对两种加速衰老小鼠（SAM）品系的两个骨骼进行组织形态计量学和实时PCR分析。SAMP8和SAMR1，具有潜在不同的骨表型。在这两种小鼠中，下颌骨颈的骨小梁体积比股骨远端干骺端高10倍。SAMR 1的股骨体积略高于SAMP 8，但下颌骨的体积则不高。COLI和胶原结合SLRP的mRNA，除了核心蛋白聚糖，在下颌骨骨髓高于股骨骨髓。尽管两种小鼠品系的股骨骨髓均不表达纤调蛋白和光蛋白聚糖，SAMR 1品系的双糖链蛋白聚糖也不表达，但它们在下颌骨骨髓中高度表达。这些分子在下颌骨中的高基因表达可能意味着该骨组织中的活跃骨形成和重塑。
Although the craniofacial and the appendicular bones develop embryologically from different origins and show different architectures and functions, it is still unclear if there exist site-specific genetic and phenotypic differences in these bones. The aim of this study was to analyze the gene expression levels of collagen-binding small leucine-rich proteoglycans (SLRPs) and type I collagen (COLI), the major matrix proteins in bone, in mouse mandibular and femoral bone marrows. Histomorphometrical and real-time PCR analyses were performed on the two bones of two senescence-accelerated mouse (SAM) strains. SAMP8 and SAMR1, with potentially different bone phenotypes. In both mice, the trabecular bone volume was 10 times higher at the mandibular neck than at the femoral distal metaphysis. The volume in the femur but not in the mandible was slightly higher in SAMR1 than in SAMP8. The mRNAs of COLI and collagen-binding SLRPs, except decorin, were higher in the mandibular bone marrow than in the femoral marrow. Although the femoral marrow did not express fibromodulin and lumican in both mouse strains and biglycan in the SAMR1 strain, they were highly expressed in the mandibular marrow. High gene expression of these molecules in the mandibular bone may imply active bone formation and remodeling in this bone tissue.
影响因子：
6.9
作者：
Matsuura, T;Duarte, WR;Yamauchi, M
通讯作者：
Yamauchi, M