A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
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DOI：
10.3390/ijms24087332
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发表时间：
2023-04-15
影响因子：
5.6
通讯作者：
Zhao, Yufen
中科院分区：
文献类型：
--
作者：
Zhao, Runjin;Huang, Biling;Lu, Gang;Fu, Songsen;Ying, Jianxi;Zhao, Yufen
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.
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影响因子：
3.5
作者：
Gałęzowska G;Ratajczyk J;Wolska L
通讯作者：
Wolska L
DOI：
10.1016/j.jchromb.2012.10.037
发表时间：
2013-01-01
影响因子：
3
作者：
Lu, Tian;Olesik, Susan V.
通讯作者：
Olesik, Susan V.
DOI：
10.1002/9780470110478.ch1
发表时间：
1981-01-01
期刊：
Methods of biochemical analysis
影响因子：
--
作者：
MacKenzie, S L
通讯作者：
MacKenzie, S L
影响因子：
2.1
作者：
Verardo, Giancarlo;Geatti, Paola;Strazzolini, Paolo
通讯作者：
Strazzolini, Paolo
DOI：
10.1016/s0021-9673(00)82032-4
发表时间：
1982-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
WATANABE, Y;IMAI, K
通讯作者：
IMAI, K