Accelerated oligosaccharide absorption and altered serum metabolites during oral glucose tolerance test in young Japanese with impaired glucose tolerance.
Accelerated oligosaccharide absorption and altered serum metabolites during oral glucose tolerance test in young Japanese with impaired glucose tolerance.
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DOI：
10.1111/jdi.12719
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发表时间：
2017-08-02
影响因子：
3.2
通讯作者：
Nomura F
中科院分区：
文献类型：
--
作者：
Miki T;Lee EY;Eguchi A;Sakurai K;Sawabe Y;Yoshida T;Saito K;Yokoh H;Ishikawa K;Yokote K;Kuzuya T;Miki E;Mori C;Nomura F
糖耐量受损(IGT)是糖尿病前期的一种亚型，具有发展为糖尿病的高风险，但其病理生理机制尚不完全清楚。在本研究中，我们通过两种类型的口服葡萄糖耐量试验(OGTTS)(D-葡萄糖[GLC]和淀粉部分水解物[PHS])来检测IGT的代谢变化，重点是血清胰岛素和代谢物。我们对99名血糖阳性(GU+；n=48)或阴性(GU−；n=51)的日本年轻人进行了两种类型的糖耐量试验(GLC/OGTT和PHS/OGTT)。糖耐量试验后，将受试者分为糖耐量正常(GU−)组(GU−/NGT；n=49)、GU+组(GU+/NGT；n=28)、糖耐量低减(IGT)组(n=12)、糖尿病(n=1)和肾性糖尿(n=9)。测定血清INT、GU、−/NGT和糖耐量异常代谢物。当PHS/OGTT期间各时间点的血清胰岛素水平以其与Glc/OGTT的比值表示时，GU−/NGT的胰岛素水平呈时间依赖性增加，而IGT则无明显变化。GU−/NGT患者的血清INS水平也有这种升高，而IGT患者则未检测到，提示IGT患者对寡糖的吸收缺乏减速。代谢组学分析表明，GU−/NGT和IGT患者血清中两种未知功能代谢产物甲基半胱氨酸和1，7-二磷酸倍半乳糖的水平存在差异。PHS/OGTT和GLC/OGTT比较显示IGT患者低聚糖吸收加快。甲基半胱氨酸和1，7-二磷酸七硫糖可作为糖代谢异常的新标记物。
Impaired glucose tolerance (IGT) is a subtype of prediabetes, a condition having high risk for development to diabetes mellitus, but its pathophysiology is not fully understood. In the present study, we examined metabolic changes in IGT by using two types (D‐glucose [Glc] and partial hydrolysate of starch [PHS]) of oral glucose tolerance tests (OGTTs), with emphasis on serum incretins and metabolites. We carried out the two types of OGTT (Glc/OGTT and PHS/OGTT) in 99 young Japanese individuals who had tested either positive (GU +; n = 48) or negative (GU −; n = 51) for glycosuria. After OGTT, they were sub‐grouped into five categories: normal glucose tolerance (NGT) in the GU − group (GU −/NGT; n = 49), NGT in the GU + group (GU +/NGT; n = 28), IGT (n = 12), diabetes mellitus (n = 1) and renal glycosuria (n = 9). Serum incretin and metabolites of GU −/NGT and IGT were then measured. When the serum insulin level at each time‐point during PHS/OGTT was expressed as its ratio relative to Glc/OGTT, it was increased time‐dependently in GU −/NGT, but not in IGT. Such an increase in the ratio was also detected of serum incretin levels in GU −/NGT, but not in IGT, suggesting a lack of deceleration of oligosaccharide absorption in IGT. Metabolome analysis showed a difference in the serum levels of two metabolites of unknown function in mammals, methylcysteine and sedoheptulose 1,7‐bisphosphate, between GU −/NGT and IGT. Comparison of PHS/OGTT and Glc/OGTT showed that oligosaccharide absorption was accelerated in IGT. Methylcysteine and sedoheptulose 1,7‐bisphosphate could be novel markers for dysregulated glucose metabolism.
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影响因子：
7.7
作者：
Ho JE;Larson MG;Vasan RS;Ghorbani A;Cheng S;Rhee EP;Florez JC;Clish CB;Gerszten RE;Wang TJ
通讯作者：
Wang TJ
影响因子：
64.8
作者：
Loftus, B;Anderson, I;Hall, N
通讯作者：
Hall, N
影响因子：
4.8
作者：
Husain, Afzal;Sato, Dan;Nozaki, Tomoyoshi
通讯作者：
Nozaki, Tomoyoshi
影响因子：
4.3
作者：
Deo, Rahul C.;Hunter, Luke;Roth, Frederick P.
通讯作者：
Roth, Frederick P.
影响因子：
8.2
作者：
Giesbertz, Pieter;Padberg, Inken;Daniel, Hannelore
通讯作者：
Daniel, Hannelore