Conidia of the insect pathogenic fungus, Metarhizium anisopliae, fail to adhere to mosquito larval cuticle.
Conidia of the insect pathogenic fungus, Metarhizium anisopliae, fail to adhere to mosquito larval cuticle.
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DOI：
10.1098/rsos.140193
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发表时间：
2014-10
影响因子：
3.5
通讯作者：
Butt TM
中科院分区：
文献类型：
--
作者：
Greenfield BP;Lord AM;Dudley E;Butt TM
昆虫病原真菌金龟子绿僵菌的分生孢子对节肢动物宿主表皮的粘附最初涉及疏水力，随后是由胞外酶的作用和粘液分泌促进的固结。利用基因表达分析和原子力显微镜直接定量测定了M.绿僵菌和埃及伊蚊的水生幼虫阶段的角质层和代表性的陆地宿主黄粉虫。基因表达数据表明，两种宿主的病原体的识别，然而，粘附到蚊子的部队比观察到的黄粉虫约低五倍。虽然弱力记录在伊蚊，绿僵菌是无法巩固坚定的附件。角质层组成的分析显示，伊蚊幼虫被认为是需要对宿主角质层的真菌发展的长链烃的情况下。据我们所知，这项研究提供了第一个证据表明，绿僵菌不能在原位与埃及伊蚊幼虫形成牢固的附着，从而阻止了正常的入侵途径和致病作用的发生。
Adhesion of conidia of the insect pathogenic fungus, Metarhizium anisopliae, to the arthropod host cuticle initially involves hydrophobic forces followed by consolidation facilitated by the action of extracellular enzymes and secretion of mucilage. Gene expression analysis and atomic force microscopy were used to directly quantify recognition and adhesion between single conidia of M. anisopliae and the cuticle of the aquatic larval stage of Aedes aegypti and a representative terrestrial host, Tenebrio molitor. Gene expression data indicated recognition by the pathogen of both hosts; however, the forces for adhesion to the mosquito were approximately five times lower than those observed for Tenebrio. Although weak forces were recorded in response to Aedes, Metarhizium was unable to consolidate firm attachment. An analysis of the cuticular composition revealed an absence of long-chain hydrocarbons in Aedes larvae which are thought to be required for fungal development on host cuticle. This study provides, to our knowledge, the first evidence that Metarhizium does not form firm attachment to Ae. aegypti larvae in situ, therefore preventing the normal route of invasion and pathogenesis from occuring.
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