Replication of the Mammalian Genome by Replisomes Specific for Euchromatin and Heterochromatin.
Replication of the Mammalian Genome by Replisomes Specific for Euchromatin and Heterochromatin.
复制标题
DOI：
10.3389/fcell.2021.729265
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Seidman MM
中科院分区：
文献类型：
--
作者：
Zhang J;Bellani MA;Huang J;James RC;Pokharel D;Gichimu J;Gali H;Stewart G;Seidman MM
Replisomes follow a schedule in which replication of DNA in euchromatin is early in S phase while sequences in heterochromatin replicate late. Impediments to DNA replication, referred to as replication stress, can stall replication forks triggering activation of the ATR kinase and downstream pathways. While there is substantial literature on the local consequences of replisome stalling–double strand breaks, reversed forks, or genomic rearrangements–there is limited understanding of the determinants of replisome stalling vs. continued progression. Although many proteins are recruited to stalled replisomes, current models assume a single species of “stressed” replisome, independent of genomic location. Here we describe our approach to visualizing replication fork encounters with the potent block imposed by a DNA interstrand crosslink (ICL) and our discovery of an unexpected pathway of replication restart (traverse) past an intact ICL. Additionally, we found two biochemically distinct replisomes distinguished by activity in different stages of S phase and chromatin environment. Each contains different proteins that contribute to ICL traverse.
登录
查看更多内容
影响因子：
16
作者：
Baretic, Domagoj;Jenkyn-Bedford, Michael;Yeeles, Joseph T. P.
通讯作者：
Yeeles, Joseph T. P.
影响因子：
8.8
作者：
Bogliolo, Massimo;Bluteau, Dominique;Surralles, Jordi
通讯作者：
Surralles, Jordi
影响因子：
16.8
作者：
Guilliam, Thomas A.;Yeeles, Joseph T. P.
通讯作者：
Yeeles, Joseph T. P.
影响因子：
56.9
作者：
Gao, Yang;Cui, Yanxiang;Yang, Wei
通讯作者：
Yang, Wei
转录复制冲突取向调节R环水平并激活不同的DNA损伤反应。
影响因子：
64.5
作者：
Hamperl S;Bocek MJ;Saldivar JC;Swigut T;Cimprich KA
通讯作者：
Cimprich KA