The effects of high-fat diet, branched-chainamino acids and exercise on female C57BL/6 mouse Achilles tendon biomechanical properties.
The effects of high-fat diet, branched-chainamino acids and exercise on female C57BL/6 mouse Achilles tendon biomechanical properties.
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DOI：
10.1302/2046-3758.29.2000196
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发表时间：
2013
影响因子：
4.6
通讯作者：
Pearson KJ
中科院分区：
文献类型：
--
作者：
Boivin GP;Platt KM;Corbett J;Reeves J;Hardy AL;Elenes EY;Charnigo RJ;Hunter SA;Pearson KJ
这项研究的目标是：1)确定高脂饮食(HFD)喂养雌性小鼠是否会对跟腱和股四头肌的生物力学和组织学后果产生负面影响；以及2)研究运动和支链氨基酸(BCAA)的补充是否会影响这些参数或减轻因摄入HFD而产生的任何负面后果。我们在雌性C57BL/6小鼠上观察了60%高脂饲料喂养16周、自愿运动(自由选择轮子跑)和支链氨基酸给药的效果。在实验结束时取出跟腱和股四头肌，并进行组织学和生物力学评估。高脂饲料喂养显著降低跟腱模数，组织学未见改变。BCAA可显著降低运动正常饮食小鼠跟腱的僵硬程度。运动部分改善了高脂饲料喂养的小鼠的体重增加和血糖水平，导致跟腱的最大负荷显著降低，股四头肌的平均纤维直径增加。体重与几个生物力学特性之间存在显著的相关性，这表明控制肥胖对于保持健康的肌腱特性的重要性。综上所述，这项研究显示肥胖和体重对肌腱生物力学性能有显著影响，而运动和支链氨基酸的影响有限。引用这篇文章：骨关节资源2013；2：186-92。
The goals of this study were: 1) to determine if high-fat diet (HFD) feeding in female mice would negatively impact biomechanical and histologic consequences on the Achilles tendon and quadriceps muscle; and 2) to investigate whether exercise and branched-chain amino acid (BCAA) supplementation would affect these parameters or attenuate any negative consequences resulting from HFD consumption. We examined the effects of 16 weeks of 60% HFD feeding, voluntary exercise (free choice wheel running) and BCAA administration in female C57BL/6 mice. The Achilles tendons and quadriceps muscles were removed at the end of the experiment and assessed histologically and biomechanically. HFD feeding significantly decreased the Achilles tendon modulus without histological alterations. BCAA administration significantly decreased the stiffness of Achilles tendons in the exercised normal diet mice. Exercise partially ameliorated both the weight gain and glucose levels in the HFD-fed mice, led to a significant decrease in the maximum load of the Achilles tendon, and an increase in the average fibril diameter of the quadriceps femoris muscle. There were significant correlations between body weight and several biomechanical properties, demonstrating the importance of controlling obesity for maintaining healthy tendon properties. In summary, this study showed a significant impact of obesity and body weight on tendon biomechanical properties with limited effects of exercise and BCAAs. Cite this article: Bone Joint Res 2013;2:186–92.
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影响因子：
3.7
作者：
Macotela Y;Emanuelli B;Bång AM;Espinoza DO;Boucher J;Beebe K;Gall W;Kahn CR
通讯作者：
Kahn CR
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4.8
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3
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Raskin, P
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3.4
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通讯作者：
van Loon, Luc J. C.
影响因子：
1.8
作者：
Faria, Aurelio;Gabriel, Ronaldo;Moreira, Helena
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