Cardiac troponin I, cardiac troponin T, and creatine kinase MB in dialysis patients without ischemic heart disease: evidence of cardiac troponin T expression in skeletal muscle.
Cardiac troponin I, cardiac troponin T, and creatine kinase MB in dialysis patients without ischemic heart disease: evidence of cardiac troponin T expression in skeletal muscle.
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无缺血性心脏病的透析患者中的心肌肌钙蛋白 I、心肌肌钙蛋白 T 和肌酸激酶 MB：骨骼肌中心肌肌钙蛋白 T 表达的证据。
DOI：
10.1093/clinchem/43.6.976
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发表时间：
1997
影响因子：
9.3
通讯作者：
S. Sharkey
中科院分区：
文献类型：
--
作者：
M. McLaurin;F. Apple;E. Voss;C. Herzog;S. Sharkey
第一代ELISA免疫测定法测定的慢性透析患者血清心肌肌钙蛋白T（cTnT）浓度经常升高，在没有急性缺血性心脏病的情况下，肌酸激酶（CK）MB质量也是如此。本研究旨在比较无急性缺血性心脏病的透析患者中心肌损伤的四种血清标志物[CK-MB质量、第一代ELISA cTnT、第二代酶联免疫cTnT和心肌肌钙蛋白I（cTnI）]。我们还评估了透析患者的骨骼肌作为血清cTnT的潜在来源。通过病史和体格检查、心电图和二维超声心动图研究，临床评价组（n = 24）中无患者有缺血性心脏病的证据。生化标志物测定系列透析前血液样本与特异性单克隆抗体为基础的免疫测定。对于几名患者，至少有一份样本测量值高于参考上限：CK-MB，7/24（30%）; ELISA cTnT，17/24（71%）; Enzymun cTnT，3/18（17%）; cTnI，1/24（4%）。在一个单独的透析患者组（n = 5），cTnT的表达，但不是cTnI，在5个骨骼肌活检的4个蛋白质印迹分析证明。无急性缺血性心脏病的慢性透析患者常出现血清CK-MB和cTnT升高。第二代cTnT（Enzymun）检测试剂的特异性优于第一代（ELISA）检测试剂; cTnI是目前可用的最具特异性的生化标志物。cTnT在透析患者的骨骼肌中表达，而cTnI不表达。
Serum cardiac troponin T (cTnT) concentrations are frequently increased in chronic dialysis patients as measured by the first-generation ELISA immunoassay, as is creatine kinase (CK) MB mass in the absence of acute ischemic heart disease. We designed this study to compare four serum markers of myocardial injury [CK-MB mass, first-generation ELISA cTnT, second-generation Enzymun cTnT, and cardiac troponin I (cTnI)] in dialysis patients without acute ischemic heart disease. We also evaluated skeletal muscle from dialysis patients as a potential source of serum cTnT. No patients in the clinical evaluation group (n = 24) studied by history and by physical examination, electrocardiography, and two-dimensional echocardiography had evidence of ischemic heart disease. Biochemical markers were measured in serial predialysis blood samples with specific monoclonal antibody-based immunoassays. For several patients at least one sample measured above the upper reference limit: CK-MB, 7 of 24 (30%); ELISA cTnT, 17 of 24 (71%); Enzymun cTnT, 3 of 18 (17%); and cTnI, 1 of 24 (4%). In a separate group of dialysis patients (n = 5), expression of cTnT, but not cTnI, was demonstrated by Western blot analysis in 4 of 5 skeletal muscle biopsies. Chronic dialysis patients without acute ischemic heart disease frequently had increased serum CK-MB and cTnT. The specificity of the second-generation cTnT (Enzymun) assay was improved over that of the first-generation (ELISA) assay; cTnI was the most specific of the currently available biochemical markers. cTnT, but not cTnI, was expressed in the skeletal muscle of dialysis patients.
揭示肾衰竭患者肌酸激酶同工酶的人为增加。
DOI：
--
发表时间：
1984
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Jaffe,AS;Ritter,C;Meltzer,V;Harter,H;Roberts,R
通讯作者：
Roberts,R
影响因子：
9.3
作者：
Jesse E. Adams;Kenneth B. Schechtman;Yvonne Landt;J. Ladenson;A. S. Jaffe
通讯作者：
Jesse E. Adams;Kenneth B. Schechtman;Yvonne Landt;J. Ladenson;A. S. Jaffe
心脏肌钙蛋白 T 同工型表达与接受先天性心脏病矫正手术的患者的病理生理学描述相关。
影响因子：
37.8
作者：
Saba,Z;Nassar,R;Ungerleider,RM;Oakeley,AE;Anderson,PA
通讯作者：
Anderson,PA