Biosynthesis of storage compounds by Rhodococcus jostii RHA1 and global identification of genes involved in their metabolism.
Biosynthesis of storage compounds by Rhodococcus jostii RHA1 and global identification of genes involved in their metabolism.
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DOI：
10.1186/1471-2164-9-600
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发表时间：
2008-12-12
期刊：
影响因子：
4.4
通讯作者：
Alvarez HM
中科院分区：
文献类型：
--
作者：
Hernández MA;Mohn WW;Martínez E;Rost E;Alvarez AF;Alvarez HM
红球菌属的成员经常在土壤和其他自然环境中发现，并且对这些环境中常见的压力具有很强的抵抗力。储存化合物的积累允许细胞在波动的环境条件下生存和代谢适应。本研究的目的是对乔氏红球菌（Rhodococcus jostii） RHA1编码多种储存化合物代谢的关键基因进行全基因组生物信息学分析，并研究其合成和积累甘油三酯（TAG）、蜡酯、聚羟基烷酸酯（PHA）、糖原和聚磷酸盐（PolyP）的能力。我们在RHA1基因组中发现：14个基因编码推测的蜡酯合成酶/酰基辅酶a：二酰基甘油酰基转移酶（WS/DGATs）可能参与TAG和蜡酯的生物合成；共有54个基因编码可能参与TAG和蜡酯降解的脂肪酶/酯酶；3组编码PHA合成酶和PHA解聚合酶的基因；6个基因编码糖原代谢的关键酶，1个基因编码推定的多磷酸激酶和3个推定的外多磷酸酶基因。在可能的情况下，鉴定上述蛋白质中的关键氨基酸残基（通常位于活性位点、效应物结合位点或底物结合位点），以支持基因鉴定。在限氮条件下生长的RHA1细胞，以TAG为主要储存化合物，再加上蜡酯、PHA（含3-羟基丁酸酯和3-羟基戊酸酯单体）、糖原和PolyP。以前已知红球菌成员会积累TAG，蜡酯，pha和polyP，但这是该属中首次报道糖原积累。RHA1具有积累多种储存化合物的关键基因。在限氮条件下，脂质是主要的储存化合物。广泛的合成和代谢储存化合物的能力似乎有助于RHA1对环境胁迫的多功能性反应。
Members of the genus Rhodococcus are frequently found in soil and other natural environments and are highly resistant to stresses common in those environments. The accumulation of storage compounds permits cells to survive and metabolically adapt during fluctuating environmental conditions. The purpose of this study was to perform a genome-wide bioinformatic analysis of key genes encoding metabolism of diverse storage compounds by Rhodococcus jostii RHA1 and to examine its ability to synthesize and accumulate triacylglycerols (TAG), wax esters, polyhydroxyalkanoates (PHA), glycogen and polyphosphate (PolyP). We identified in the RHA1 genome: 14 genes encoding putative wax ester synthase/acyl-CoA:diacylglycerol acyltransferase enzymes (WS/DGATs) likely involved in TAG and wax esters biosynthesis; a total of 54 genes coding for putative lipase/esterase enzymes possibly involved in TAG and wax ester degradation; 3 sets of genes encoding PHA synthases and PHA depolymerases; 6 genes encoding key enzymes for glycogen metabolism, one gene coding for a putative polyphosphate kinase and 3 putative exopolyphosphatase genes. Where possible, key amino acid residues in the above proteins (generally in active sites, effectors binding sites or substrate binding sites) were identified in order to support gene identification. RHA1 cells grown under N-limiting conditions, accumulated TAG as the main storage compounds plus wax esters, PHA (with 3-hydroxybutyrate and 3-hydroxyvalerate monomers), glycogen and PolyP. Rhodococcus members were previously known to accumulate TAG, wax esters, PHAs and polyP, but this is the first report of glycogen accumulation in this genus. RHA1 possess key genes to accumulate diverse storage compounds. Under nitrogen-limiting conditions lipids are the principal storage compounds. An extensive capacity to synthesize and metabolize storage compounds appears to contribute versatility to RHA1 in its responses to environmental stresses.
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影响因子：
3.4
作者：
Chen, Shiqiong;Liu, Jingfang;Xiang, Hua
通讯作者：
Xiang, Hua
影响因子：
3.2
作者：
Daniel, J;Deb, C;Kolattukudy, PE
通讯作者：
Kolattukudy, PE
影响因子：
3.2
作者：
ELBEIN, AD;MITCHELL, M
通讯作者：
MITCHELL, M
影响因子：
4.4
作者：
Banchio, C;Gramajo, H
通讯作者：
Gramajo, H
DOI：
10.1016/s0964-8305(02)00120-8
发表时间：
2003-01-01
影响因子：
4.8
作者：
Alvarez, HM
通讯作者：
Alvarez, HM