Mechanoenzymatic reactions for the hydrolysis of PET.
Mechanoenzymatic reactions for the hydrolysis of PET.
复制标题
PET水解的机械酶反应。
作者：
近年来，聚对苯二甲酸乙二酯(PET)的酶降解研究取得了新的进展，开发出了许多PET水解酶和突变体。随着自然界中聚酯数量的增加，迫切需要开发可扩展的方法，将聚合物分解成其单体，以供回收或其他用途。机械酶催化反应作为传统生物催化反应的一种绿色高效的替代方法，近年来得到了广泛的关注。我们第一次报告了，与典型的基于溶液的反应相比，通过使用反应老化的球磨循环，全细胞PETase酶降解PET的产量增加了27倍。与该领域的其他主要降解反应相比，这种方法导致所需溶剂减少了2600倍，与报道的工业规模的PET水解反应相比，减少了30倍。描述了使用全细胞PETase降解不同的PET材料的机械酶反应。
Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions. Mechanoenzymatic reactions are described for the degradation of different PET materials using whole cell PETases.
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影响因子：
13.6
作者：
Geyer R;Jambeck JR;Law KL
通讯作者：
Law KL
DOI：
10.1002/anie.202203061
发表时间：
2022-09-12
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Eiamthong B;Meesawat P;Wongsatit T;Jitdee J;Sangsri R;Patchsung M;Aphicho K;Suraritdechachai S;Huguenin-Dezot N;Tang S;Suginta W;Paosawatyanyong B;Babu MM;Chin JW;Pakotiprapha D;Bhanthumnavin W;Uttamapinant C
通讯作者：
Uttamapinant C
影响因子：
5.6
作者：
通讯作者：
--
影响因子：
3.1
作者：
Friscic, Tomislav;Childs, Scott L.;Jones, William
通讯作者：
Jones, William
对聚乙二醇乙酸乙酯）降解的分子机制的结构洞察力。
影响因子：
16.6
作者：
Joo S;Cho IJ;Seo H;Son HF;Sagong HY;Shin TJ;Choi SY;Lee SY;Kim KJ
通讯作者：
Kim KJ