The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria.
The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria.
复制标题
移动遗传元件在有症状和无症状大肠杆菌菌尿病例毒力因子携带中的作用。
DOI：
10.1128/spectrum.04710-22
复制
发表时间：
2023-06-15
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
尿路致病性大肠杆菌（UPEC）具有极其多样的基因型和表型。单个菌株可以不同地携带不同的毒力因子，这使得定义这种病原体的分子特征具有挑战性。对于许多细菌性病原体，移动遗传因子（MGEs）构成了毒力因子获取的主要机制。对于尿中大肠杆菌，MGEs的总分布及其在获得毒力因子中的作用尚不明确，包括在有症状感染与无症状细菌尿（ASB）的情况下。在这项工作中，我们鉴定了151株分离自尿路感染（UTI）或ASB患者的大肠杆菌。对于这两组大肠杆菌，我们对质粒、噬菌体和转座子的存在进行了分类。我们分析了MGE序列中毒力因子和耐药基因的存在。这些MGEs仅与约4%的总毒力相关基因相关，而质粒则与约15%的耐药基因相关。我们的分析表明，在大肠杆菌菌株中，MGEs不是尿路发病和症状性感染的主要驱动因素。重要性：大肠杆菌是尿路感染最常见的病原体，与尿路感染相关的菌株被称为“尿路致病性”大肠杆菌或UPEC。在泌尿系大肠杆菌中，MGEs的全球格局及其与毒力因子携带和临床症状的关系需要更明确。在这里，我们证明了许多假定的UPEC毒力因素与MGEs引起的获得无关。目前的工作增强了我们对尿相关大肠杆菌菌株间变异性和致病潜力的理解，并指出区分ASB和UTI分离株的更微妙的基因组差异。
Uropathogenic Escherichia coli (UPEC) is extremely diverse genotypically and phenotypically. Individual strains can variably carry diverse virulence factors, making it challenging to define a molecular signature for this pathotype. For many bacterial pathogens, mobile genetic elements (MGEs) constitute a major mechanism of virulence factor acquisition. For urinary E. coli, the total distribution of MGEs and their role in the acquisition of virulence factors is not well defined, including in the context of symptomatic infection versus asymptomatic bacteriuria (ASB). In this work, we characterized 151 isolates of E. coli, derived from patients with either urinary tract infection (UTI) or ASB. For both sets of E. coli, we catalogued the presence of plasmids, prophage, and transposons. We analyzed MGE sequences for the presence of virulence factors and antimicrobial resistance genes. These MGEs were associated with only ~4% of total virulence associated genes, while plasmids contributed to ~15% of antimicrobial resistance genes under consideration. Our analyses suggests that, across strains of E. coli, MGEs are not a prominent driver of urinary tract pathogenesis and symptomatic infection. IMPORTANCE Escherichia coli is the most common etiological agent of urinary tract infections (UTIs), with UTI-associated strains designated “uropathogenic” E. coli or UPEC. Across urinary strains of E. coli, the global landscape of MGEs and its relationship to virulence factor carriage and clinical symptomatology require greater clarity. Here, we demonstrate that many of the putative virulence factors of UPEC are not associated with acquisition due to MGEs. The current work enhances our understanding of the strain-to-strain variability and pathogenic potential of urine-associated E. coli and points toward more subtle genomic differences distinguishing ASB from UTI isolates.
登录
查看更多内容
影响因子：
4.4
作者：
Aertsen, A;Faster, D;Michiels, CW
通讯作者：
Michiels, CW
影响因子：
5.1
作者：
Clermont, Olivier;Condamine, Benedicte;Denamur, Erick
通讯作者：
Denamur, Erick
影响因子：
9.4
作者：
Boerlin, P;McEwen, SA;Gyles, CL
通讯作者：
Gyles, CL
DOI：
10.1093/jac/dkaa345
发表时间：
2020-12-01
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Bortolaia V;Kaas RS;Ruppe E;Roberts MC;Schwarz S;Cattoir V;Philippon A;Allesoe RL;Rebelo AR;Florensa AF;Fagelhauer L;Chakraborty T;Neumann B;Werner G;Bender JK;Stingl K;Nguyen M;Coppens J;Xavier BB;Malhotra-Kumar S;Westh H;Pinholt M;Anjum MF;Duggett NA;Kempf I;Nykäsenoja S;Olkkola S;Wieczorek K;Amaro A;Clemente L;Mossong J;Losch S;Ragimbeau C;Lund O;Aarestrup FM
通讯作者：
Aarestrup FM
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.