Mechanisms of coronavirus cell entry mediated by the viral spike protein.
Mechanisms of coronavirus cell entry mediated by the viral spike protein.
复制标题
DOI：
10.3390/v4061011
复制
发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Whittaker GR
中科院分区：
文献类型：
--
作者：
Belouzard S;Millet JK;Licitra BN;Whittaker GR
冠状病毒是一种包膜的正链RNA病毒，在细胞质中复制。为了将核衣壳送入宿主细胞，它们依赖于其包膜与宿主细胞膜的融合。刺激性糖蛋白(S)介导病毒入侵，是细胞嗜性和致病的主要决定因素。它被归类为I类融合蛋白，负责与宿主细胞上的受体结合，并介导宿主和病毒膜的融合-这一过程由S蛋白的主要构象变化驱动。本文讨论了冠状病毒的入侵机制，重点讨论了冠状病毒启动S蛋白构象变化的不同触发因素：受体结合、低pH暴露和蛋白水解性激活。我们还强调了冠状病毒S蛋白与其他I类病毒融合蛋白的共性，以及赋予冠状病毒不同嗜性、致病性和宿主跨物种传播特征的独特特征。
Coronaviruses are enveloped positive-stranded RNA viruses that replicate in the cytoplasm. To deliver their nucleocapsid into the host cell, they rely on the fusion of their envelope with the host cell membrane. The spike glycoprotein (S) mediates virus entry and is a primary determinant of cell tropism and pathogenesis. It is classified as a class I fusion protein, and is responsible for binding to the receptor on the host cell as well as mediating the fusion of host and viral membranes—A process driven by major conformational changes of the S protein. This review discusses coronavirus entry mechanisms focusing on the different triggers used by coronaviruses to initiate the conformational change of the S protein: receptor binding, low pH exposure and proteolytic activation. We also highlight commonalities between coronavirus S proteins and other class I viral fusion proteins, as well as distinctive features that confer distinct tropism, pathogenicity and host interspecies transmission characteristics to coronaviruses.
登录
查看更多内容
DOI：
10.1074/jbc.m110.103275
发表时间：
2010-07-23
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Belouzard S;Madu I;Whittaker GR
通讯作者：
Whittaker GR
影响因子：
5.4
作者：
Glowacka, Ilona;Bertram, Stephanie;Poehlmann, Stefan
通讯作者：
Poehlmann, Stefan
影响因子：
5.4
作者：
GALLAGHER, TM;ESCARMIS, C;BUCHMEIER, MJ
通讯作者：
BUCHMEIER, MJ
影响因子：
5.4
作者：
Bosch, Berend Jan;Bartelink, Willem;Rottier, Peter J. M.
通讯作者：
Rottier, Peter J. M.
DOI：
10.1073/pnas.90.5.1716
发表时间：
1993-03-01
影响因子：
11.1
作者：
DVEKSLER, GS;PENSIERO, MN;HOLMES, KV
通讯作者：
HOLMES, KV