Disruption of the retinal basal lamina during early embryonic development leads to a retraction of vitreal end feet, an increased number of ganglion cells, and aberrant axonal outgrowth
Disruption of the retinal basal lamina during early embryonic development leads to a retraction of vitreal end feet, an increased number of ganglion cells, and aberrant axonal outgrowth
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早期胚胎发育过程中视网膜基底层的破坏导致玻璃体末端足回缩、神经节细胞数量增加和轴突异常生长
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
W. Halfter
中科院分区：
文献类型：
--
作者：
W. Halfter
将细菌型胶原酶注射到雏鸡和鹌鹑胚胎的玻璃体中。免疫细胞化学和超微结构研究表明，胶原酶溶解了注射眼的视网膜基底膜。注射酶后1小时可首次检测到基底板断裂，并在3小时内完成。随着进一步的发展，视网膜基板没有重建；然而，外围视网膜中新发展的神经上皮产生了一个完整的基板。Western印迹分析表明，梭状芽胞杆菌胶原酶能降解多种胶原蛋白，但不降解非胶原蛋白。胚胎3~6天视网膜基膜断裂导致神经上皮细胞的末端足回缩，胰岛-1+细胞(最可能是神经节细胞)数量增加，光纤层厚度增加，视神经轴突向视盘方向异常生长。当视网膜基底板在发育后期被破坏时，这些变化都没有观察到。目前的数据表明，视网膜基板通过将神经上皮细胞锚定在视网膜的软膜表面，在该结构的正常细胞结构的发育中起着重要的作用。有人认为，视网膜玻璃体部分细胞外环境的改变，导致神经上皮细胞末端足的回缩，是导致胰岛-1+细胞数量增加和轴突导航异常的原因。J.Comp.神经。397：89-104,1998。©1998 Wiley-Liss，Inc.
Bacterial collagenase was injected into the vitreous of the eye of chick and quail embryos. Immunocytochemical and ultrastructural studies revealed that the collagenase dissolved the retinal basal lamina of the injected eye. The basal lamina disruption was first detectable 1 hour after enzyme injection and was complete within 3 hours. With further development, the retinal basal lamina was not reestablished; newly developing neuroepithelium in the peripheral retina, however, generated an intact basal lamina. Western blot analysis showed that Clostridial collagenase degraded various collagens but spared noncollagenous proteins. Basal lamina disruption of embryonic day 3 to 6 retinae led to the retraction of the end feet of the neuroepithelial cells, caused an increase in the number of Islet‐1+ cells (most likely ganglion cells), an increase in the thickness of the optic fiber layer, and aberrant growth of optic axons on their way toward the optic disc. None of these changes were observed when retinal basal laminae were disrupted at later stages of development. The present data demonstrate that the retinal basal lamina, by anchoring the neuroepithelial cells to the pial surface of the retina, has an important function in the development of the normal cytoarchitecture of this structure. It is proposed that the altered extracellular environment in the vitreal part of the retina, resulting in the retraction of the neuroepithelial end feet, is responsible for the increased number of Islet‐1+ cells and the aberrant axonal navigation. J. Comp. Neurol. 397:89–104, 1998. © 1998 Wiley‐Liss, Inc.
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DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
发育中的禽类初级角膜基质和玻璃体获得 IX 型胶原蛋白。
影响因子：
2.7
作者：
Fitch,JM;Mentzer,A;Mayne,R;Linsenmayer,TF
通讯作者：
Linsenmayer,TF
影响因子：
2.9
作者：
BOND, MD;VANWART, HE
通讯作者：
VANWART, HE
II 型和 IX 型胶原蛋白在上皮间质界面的独立沉积。
DOI：
10.1242/dev.105.1.85
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Fitch,JM;Mentzer,A;Mayne,R;Linsenmayer,TF
通讯作者：
Linsenmayer,TF
DOI：
--
发表时间：
1981-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
通讯作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung