The nature of the fungal cargo induces significantly different temporal programmes of macrophage phagocytosis.
The nature of the fungal cargo induces significantly different temporal programmes of macrophage phagocytosis.
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DOI：
10.1016/j.tcsw.2022.100082
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Gow, Neil A. R.
中科院分区：
文献类型：
--
作者：
Alonso, Maria Fernanda;Bain, Judith M.;Rudkin, Fiona M.;Erwig, Lars P.;Brown, Alistair J. P.;Gow, Neil A. R.
巨噬细胞摄取真菌细胞的速率在不同病原真菌物种之间变化高达26倍。对于不同的病原真菌，巨噬细胞的吞噬体酸化时间变化多达29倍。热杀伤以种属依赖性方式影响与巨噬细胞相互作用的动力学。吞噬作用是我们对真菌病原体的免疫防御的重要组成部分。吞噬细胞迁移、识别、摄取和吞噬溶酶体成熟的动力学差异取决于真菌货物的特性，特别是细胞壁组成和细胞形态的差异。然而，专注于吞噬细胞与单个真菌物种的相互作用的研究并没有使这些不同物种之间的这些相互作用的动力学进行比较。因此，我们使用活细胞视频显微镜检查的时间动态的一系列真菌货物的巯基乙酸盐引起的腹腔巨噬细胞从C57 BL/6小鼠的吞噬作用。同时用白色念珠菌、光滑念珠菌、酿酒酵母和新型隐球菌（野生型和无囊突变体cap 59 Δ）的酵母细胞以及烟曲霉和卷状毛霉的孢子挑战均匀的巨噬细胞群，以使标准化的比较相互作用能够从吞噬作用的不同阶段定量。真菌细胞的吸收率差异高达26倍，而诱导吞噬体酸化的时间差异高达29倍。热杀死或调理真菌的目标显着影响的动力学的相互作用，在一个物种特异性的方式。真菌和巨噬细胞杀伤试验进一步揭示了货物特异性的吞噬作用差异和真菌逃避机制的多样性。因此，同时评估巨噬细胞与不同真菌病原体的相互作用，突出了真菌-吞噬细胞相互作用过程中动力学和生长反应的主要差异，这些差异可能会影响发病机制和毒力。
The rate of uptake of fungal cells by macrophages varies by up to 26-fold between different pathogenic fungal species. Phagosome acidification times for macrophages varies by as much as 29-fold for different pathogenic fungi. Heat-killing affects the kinetics of the interaction with macrophages in a species-dependent manner. Phagocytosis is an essential component of our immune defence against fungal pathogens. Differences in the dynamics of phagocyte migration, recognition, uptake and phagolysosome maturation are dependent on the characteristics of the fungal cargo, and in particular to differences in cell wall composition and cellular morphology. However, studies that have focused on phagocyte interactions with individual fungal species have not enabled comparisons in the kinetics of these interactions to be made between these different species. We therefore used live cell video microscopy to examine the temporal dynamics of phagocytosis for a range of fungal cargoes by thioglycollate–elicited peritoneal macrophages from C57BL/6 mice. Uniform populations of macrophages were challenged at the same time with yeast cells of Candida albicans, Candida glabrata, Saccharomyces cerevisiae and Cryptococcus neoformans (wild–type and an acapsular mutant, cap59Δ), and spores of Aspergillus fumigatus and Mucor circinelloides to enable standardized comparative interactions to be quantified from different stages of phagocytosis. Differences in the rate of uptake of fungal cells varied by up to 26–fold, whilst differences in time to induce phagosome acidification varied by as much as 29–fold. Heat-killing or opsonizing the fungal targets markedly affected the kinetics of the interaction in a species–specific manner. Fungal and macrophage killing assays further revealed cargo–specific differences in phagocytosis and diversity in fungal evasion mechanisms. Therefore, simultaneous assessment of the interaction of macrophages with different fungal pathogens highlighted major differences in the kinetics and growth responses during fungus–phagocyte interactions that are likely to impact on pathogenesis and virulence.
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DOI：
10.1038/s41575-021-00541-2
发表时间：
2022-03
期刊：
Nature reviews. Gastroenterology & hepatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
28.3
作者：
通讯作者：
--
影响因子：
28.3
作者：
Ballou ER;Avelar GM;Childers DS;Mackie J;Bain JM;Wagener J;Kastora SL;Panea MD;Hardison SE;Walker LA;Erwig LP;Munro CA;Gow NA;Brown GD;MacCallum DM;Brown AJ
通讯作者：
Brown AJ
DOI：
10.1086/523110
发表时间：
2007-11-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Gow NA;Netea MG;Munro CA;Ferwerda G;Bates S;Mora-Montes HM;Walker L;Jansen T;Jacobs L;Tsoni V;Brown GD;Odds FC;Van der Meer JW;Brown AJ;Kullberg BJ
通讯作者：
Kullberg BJ
影响因子：
5.2
作者：
Godoy, Pablo;Darlington, Peter John;Whiteway, Malcolm
通讯作者：
Whiteway, Malcolm