Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma.
Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma.
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DOI：
10.1267/ahc.16020
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发表时间：
2016-11-01
影响因子：
2.4
通讯作者：
Nemoto N
中科院分区：
文献类型：
--
作者：
Murakami E;Nakanishi Y;Hirotani Y;Ohni S;Tang X;Masuda S;Enomoto K;Sakurai K;Amano S;Yamada T;Nemoto N
乳腺癌由于肿瘤细胞侵袭和转移，预后较差。虽然Ras同源物（Rho） A参与肿瘤细胞侵袭，但其在乳腺癌中的作用尚不清楚。本研究检测了RhoA在浸润性导管癌（IDC）中的表达，重点研究了其与表皮-间充质转化（EMT）和集体细胞侵袭的关系。获得44例手术IDC组织样本和2例正常乳腺组织样本。免疫组织化学分析RhoA、E-cadherin、vimentin和F-actin蛋白的表达。RhoA、ROCK、mTOR、AKT1和PIK3CA mRNA的表达采用激光显微解剖和半巢式定量逆转录聚合酶链反应。RhoA在肿瘤界面的表达强于肿瘤中心（P<0.001）。RhoA表达仅在her2亚型IDC中与ROCK表达相关（P<0.05）。在共表达RhoA和ROCK的idc中，F-actin在肿瘤界面上的表达强于ROCK阴性idc (P<0.0001)，尤其是在肿瘤细胞边缘。综上所述，IDC中RhoA的表达与EMT无关，而F-actin的表达增强则定位于共表达ROCK的肿瘤细胞边缘。RhoA/ROCK信号可能与集体细胞侵袭有关，特别是在her2亚型IDC中。
Breast cancer has a poor prognosis owing to tumor cell invasion and metastasis. Although Ras homolog (Rho) A is involved in tumor cell invasion, its role in breast carcinoma is unclear. Here, RhoA expression was examined in invasive ductal carcinoma (IDC), with a focus on its relationships with epidermal-mesenchymal transition (EMT) and collective cell invasion. Forty-four surgical IDC tissue samples and two normal breast tissue samples were obtained. RhoA, E-cadherin, vimentin, and F-actin protein expression were analyzed by immunohistochemistry. RhoA, ROCK, mTOR, AKT1, and PIK3CA mRNA expression were conducted using laser microdissection and semi-nested quantitative reverse transcription-polymerase chain reaction. RhoA expression was stronger on the tumor interface of IDCs than the tumor center (P<0.001). RhoA expression was correlated with ROCK expression only in HER2-subtype IDC (P<0.05). In IDCs co-expressing RhoA and ROCK, F-actin expression was stronger on the tumor interface, particularly at the edges of tumor cells, than it was in ROCK-negative IDCs (P<0.0001). In conclusion, RhoA expression was not correlated with EMT in IDC, but enhanced F-actin expression was localized on the edge of tumor cells that co-expressed ROCK. RhoA/ROCK signaling may be associated with collective cell invasion, particularly in HER2-subtype IDC.
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影响因子：
--
作者：
Lin HH;Lin HK;Lin IH;Chiou YW;Chen HW;Liu CY;Harn HI;Chiu WT;Wang YK;Shen MR;Tang MJ
通讯作者：
Tang MJ
影响因子：
3.8
作者：
Pasquier J;Abu-Kaoud N;Abdesselem H;Madani A;Hoarau-Véchot J;Thawadi HA;Vidal F;Couderc B;Favre G;Rafii A
通讯作者：
Rafii A
影响因子：
16.6
作者：
Heemskerk N;Schimmel L;Oort C;van Rijssel J;Yin T;Ma B;van Unen J;Pitter B;Huveneers S;Goedhart J;Wu Y;Montanez E;Woodfin A;van Buul JD
通讯作者：
van Buul JD
影响因子：
11.5
作者：
Mazieres, J;Antonia, T;Favre, G
通讯作者：
Favre, G
影响因子：
50.5
作者：
Ferlay, J.;Autier, P.;Boyle, P.
通讯作者：
Boyle, P.