Structural studies of gangliosides by fast atom bombardment ionization, low-energy collision-activated dissociation, and tandem mass spectrometry.
Structural studies of gangliosides by fast atom bombardment ionization, low-energy collision-activated dissociation, and tandem mass spectrometry.
复制标题
通过快原子轰击电离、低能碰撞激活解离和串联质谱法对神经节苷脂进行结构研究。
作者：
T. Kasama;S. Handa
采用负离子快原子轰击、低能碰撞激活解离和串联质谱技术对神经节苷脂进行了结构解析。在混合物分析中，通过选择单个分子离子或片段离子来简化质谱，避免了液体基质背景信号的干扰。引入碰撞激活解离产生了丰富的碎片离子，便于结构分析。在子扫描模式下，离子是由糖苷键的切割产生的，而不是由糖环的切割产生的。这些离子都含有神经酰胺部分，除了唾液酸片段离子。在亲本扫描模式下，在糖环上切割产生的产物离子被检测到在糖苷键上切割产生的离子旁边，低聚糖片段的离子也被检测到。在基于唾液酸离子的神经节苷脂的父扫描模式光谱中，所有离子都含有唾液酸残基，并且观察到的离子与高能碰撞激活解离子扫描模式中获得的离子相似。这些结果表明，结合快速原子轰击质谱和高能碰撞激活解离质谱，低能碰撞激活解离串联质谱在子扫描和父扫描模式下分析神经节苷脂结构的有效性。
Negative ion fast atom bombardment, low-energy collision-activated dissociation, and tandem mass spectrometry techniques were applied for the structural elucidation of gangliosides. The mass spectra were simplified by selecting a single molecular ion or fragment ion in the analysis of mixtures, and interference by background signals from the liquid matrix could be avoided. Introduction of collision-activated dissociation produced abundant fragment ions convenient for structural analysis. In the daughter scan mode, ions were produced by cleavage of the glycosidic bonds, and not by cleavage at the sugar ring. These ions all contain ceramide moieties, except the sialic acid fragment ion. In the parent scan mode, product ions resulting from cleavage at the sugar ring were detected beside the ions resulting from cleavage at the glycosidic bonds, and ions of oligosaccharide fragments were also detected. In parent scan mode spectra of gangliosides based on the sialic acid ion, all ions contained a sialic acid residue, and the observed ions were similar to those obtained in the high-energy collision-activated dissociation daughter scan mode. These results indicate the usefulness of low-energy collision-activated dissociation tandem mass spectrometry in the daughter and parent scan modes for the analysis of ganglioside structure, in combination with fast atom bombardment mass spectrometry and high-energy collision-activated dissociation mass spectrometry.
神经节三酰神经酰胺 (Gg3) 和唾液酸基乳糖神经酰胺 (GM3) 之间的特异性相互作用作为淋巴瘤和黑色素瘤细胞之间特异性细胞识别的基础。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kojima,N;Hakomori,S
通讯作者：
Hakomori,S
影响因子：
2.9
作者：
DOMON, B;COSTELLO, CE
通讯作者：
COSTELLO, CE