A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
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DOI：
10.3389/fbioe.2022.912073
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发表时间：
2022
影响因子：
5.7
通讯作者：
Murfee, Walter L.
中科院分区：
文献类型：
--
作者：
Lampejo, Arinola O.;Hu, Nien-Wen;Lucas, Daniela;Lomel, Banks M.;Nguyen, Christian M.;Dominguez, Carmen C.;Ren, Bing;Huang, Yong;Murfee, Walter L.
体外和体内测试之间的差距启发了仿生模型的发展。试图模拟微血管网络复杂性的组织工程模型已经成为研究细胞-细胞和细胞-环境相互作用的工具，这些相互作用在体内可能不容易观察到。然而，模型开发中的一个关键挑战是确定如何重建真实网络环境中的多细胞/系统功能复杂性，该网络环境集成了内皮细胞、平滑肌细胞、血管周细胞、淋巴管、神经、液体流动、细胞外基质和炎症细胞。这篇小型综述的目的是概述用于研究微血管动力学的流行仿生建模方法的最新进展。一个特别的重点将突出所需的工程设计要求，以匹配生理功能和保持复杂性的自上而下组织培养方法的潜力。总体而言，生理学验证、基础科学发现和治疗评估研究的例子将强调组织培养模型和仿生模型开发方法的价值，这些方法填补了体外和体内分析之间的空白，并指导血管生物学家和生理学家如何考虑微循环。
The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo. A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between in vitro and in vivo assays and guide how vascular biologists and physiologists might think about the microcirculation.
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