Yeast as a cell factory: current state and perspectives.
Yeast as a cell factory: current state and perspectives.
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DOI：
10.1186/s12934-015-0281-x
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发表时间：
2015-06-30
影响因子：
6.4
通讯作者：
Petrovič U
中科院分区：
文献类型：
--
作者：
Kavšček M;Stražar M;Curk T;Natter K;Petrovič U
酿酒酵母是生物技术中最古老、最常用的微生物之一，在散装化学品和精细化学品的生产中都有成功的应用。然而，酵母研究人员面临着进一步从旧的主力向现代细胞工厂转变的挑战，以满足下一代生物工艺的要求。用于这一开发的许多原理和工具都源自合成生物学领域，并且工程菌株确实是合成生物体。我们概述了这一转变的最重要方面，并重点介绍了近年来取得的成就以及酵母目前落后的趋势。这些方面包括：增强酵母的底物谱，重点是可再生原料的有效利用，通过生成独立电路来维持氧化还原平衡和常见碳构件的生物合成来增强产物谱，通过改进的基因组编辑和正交启动子进行精确的途径控制的要求，以及提高酵母对特定胁迫条件的耐受性。未来酵母细胞工厂所需性状的致病遗传元件将被组装成遗传模块，以便在菌株之间快速转移。这些发展将受益于生物计算方法的进步，允许整合不同类型的数据集和算法，以及基因组编辑的快速进步，这将使整个异源途径的多重靶向整合成为可能。总体目标是提供一组独立工作的模块和电路，可以根据具体情况随意组合，并为给定的生产过程产生最佳的合成宿主。
The yeast Saccharomyces cerevisiae is one of the oldest and most frequently used microorganisms in biotechnology with successful applications in the production of both bulk and fine chemicals. Yet, yeast researchers are faced with the challenge to further its transition from the old workhorse to a modern cell factory, fulfilling the requirements for next generation bioprocesses. Many of the principles and tools that are applied for this development originate from the field of synthetic biology and the engineered strains will indeed be synthetic organisms. We provide an overview of the most important aspects of this transition and highlight achievements in recent years as well as trends in which yeast currently lags behind. These aspects include: the enhancement of the substrate spectrum of yeast, with the focus on the efficient utilization of renewable feedstocks, the enhancement of the product spectrum through generation of independent circuits for the maintenance of redox balances and biosynthesis of common carbon building blocks, the requirement for accurate pathway control with improved genome editing and through orthogonal promoters, and improvement of the tolerance of yeast for specific stress conditions. The causative genetic elements for the required traits of the future yeast cell factories will be assembled into genetic modules for fast transfer between strains. These developments will benefit from progress in bio-computational methods, which allow for the integration of different kinds of data sets and algorithms, and from rapid advancement in genome editing, which will enable multiplexed targeted integration of whole heterologous pathways. The overall goal will be to provide a collection of modules and circuits that work independently and can be combined at will, depending on the individual conditions, and will result in an optimal synthetic host for a given production process.
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影响因子：
6.3
作者：
Demeke MM;Dumortier F;Li Y;Broeckx T;Foulquié-Moreno MR;Thevelein JM
通讯作者：
Thevelein JM
影响因子：
3.7
作者：
Blount BA;Weenink T;Vasylechko S;Ellis T
通讯作者：
Ellis T
DOI：
10.1007/s11693-013-9106-6
发表时间：
2013-06-01
期刊：
Systems and synthetic biology
影响因子：
--
作者：
Delgado, Ana;Porcar, Manuel
通讯作者：
Porcar, Manuel
影响因子：
4.1
作者：
Hong, Jiefang;Yang, Huajun;Zhang, Minhua
通讯作者：
Zhang, Minhua
影响因子：
4.7
作者：
DiCarlo, James E.;Conley, Andrew J.;Penttila, Merja;Jantti, Jussi;Wang, Harris H.;Church, George M.
通讯作者：
Church, George M.