The nuclear localization signal-mediated nuclear targeting of herpes simplex virus 1 early protein UL2 is important for efficient viral production
The nuclear localization signal-mediated nuclear targeting of herpes simplex virus 1 early protein UL2 is important for efficient viral production
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单纯疱疹病毒 1 早期蛋白 UL2 的核定位信号介导的核靶向对于有效的病毒生产非常重要
DOI：
10.18632/aging.102786
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发表时间：
2020-02
期刊：
影响因子：
5.2
通讯作者：
Cai Mingsheng
中科院分区：
文献类型：
--
作者：
Li Meili;Zou Xingmei;Wang Yuanfang;Xu Zuo;Ou Xiaowen;Li Yiwen;Liu Delong;Guo Yingjie;Deng Yangxi;Jiang Si;Li Tong;Shi Shaoxuan;Bao Yilong;Peng Tao;Cai Mingsheng
单纯疱疹病毒1型（HSV - 1）是一种具有代表性的α疱疹病毒，它可引发人类一系列严重疾病，但其确切的发病机制尚未完全清楚。UL2是一种参与HSV - 1 DNA复制过程的尿嘧啶 - DNA糖基化酶。
Herpes simplex virus 1 (HSV-1) is a representative alphaherpesvirus that can provoke a series of severe diseases to human being, but its exact pathogenesis is not perfectly understood. UL2, a uracil-DNA glycosylase involved in the process of HSV-1 DNA replication, has been shown to be predominantly targeted to the nuclei in our previous study, yet little is established regarding the subcellular localization signal or its related function of UL2 during HSV-1 propagation. Here, by creating a number of UL2 variants merged with enhanced yellow fluorescent protein, an authentic nuclear localization signal (NLS) of UL2 was, for the first time, identified and profiled to amino acids (aa) 1 to 17 (MKRACSRSPSPRRRPSS), and 12RRR14 was indispensable for its nuclear accumulation. Besides, the predicted nuclear export signal (aa 225 to 240) of UL2 was determined to be nonfunctional. Based on the HSV-1 bacterial artificial chromosome and homologous recombination technique, three recombinant viruses with mutations of the identified NLS, deletion and revertant of UL2 were constructed to assess the effect of UL2 nuclear targeting on HSV-1 replication. Compared to the wild type HSV-1, UL2 deletion remarkably restrained viral production, and mutation of NLS targeting UL2 to cytoplasm (pan-cellular distribution) in recombinant virus-infected cells showed a certain degree of deficiency in HSV-1 proliferation. Moreover, recombinant virus with UL2 deletion exhibited serious damages of viral DNA synthesis and mRNA expression, and these processes were partially disrupted in the recombinant virus with UL2 NLS mutation. Collectively, we had established a functional NLS in UL2 and showed that the NLS-mediated nuclear translocation of UL2 was important for efficient production of HSV-1. These data were of significance for further clarifying the biological function of UL2 during HSV-1 infection.
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影响因子：
3.7
作者：
Ranneberg-Nilsen T;Rollag H;Slettebakk R;Backe PH;Olsen Ø;Luna L;Bjørås M
通讯作者：
Bjørås M
探讨 PRV ICP22 的核输入信号和核运输分子决定因素
影响因子：
7.5
作者：
Cai M;Jiang S;Zeng Z;Li X;Mo C;Yang Y;Chen C;Xie P;Bian Y;Wang J;Huang J;Chen D;Peng T;Li M
通讯作者：
Li M
影响因子：
5.4
作者：
Lu, Chih-Chung;Huang, Ho-Ting;Chen, Mei-Ru
通讯作者：
Chen, Mei-Ru
影响因子：
3.9
作者：
Meili Li;Si Jiang;C. Mo;Z. Zeng;Xiaowei Li;Chunke Chen;Yanjia Yang;Jin-lin Wang;Jinlu Huang-Jinlu-Hua
通讯作者：
Meili Li;Si Jiang;C. Mo;Z. Zeng;Xiaowei Li;Chunke Chen;Yanjia Yang;Jin-lin Wang;Jinlu Huang-Jinlu-Hua
DOI：
10.1007/978-1-60761-652-8_30
发表时间：
2010-01-01
期刊：
IN VITRO MUTAGENESIS PROTOCOLS, THIRD EDITION
影响因子：
--
作者：
Tischer, B. Karsten;Smith, Gregory A.;Osterrieder, Nikolaus
通讯作者：
Osterrieder, Nikolaus