193 nm ultraviolet photodissociation mass spectrometry for the structural elucidation of lipid A compounds in complex mixtures.
193 nm ultraviolet photodissociation mass spectrometry for the structural elucidation of lipid A compounds in complex mixtures.
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DOI：
10.1021/ac403796n
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发表时间：
2014-02-18
影响因子：
7.4
通讯作者：
Brodbelt, Jennifer S.
中科院分区：
文献类型：
--
作者：
O'Brien, John P.;Needham, Brittany D.;Henderson, Jeremy C.;Nowicki, Emily M.;Trent, M. Stephen;Brodbelt, Jennifer S.
在这里，我们将紫外光解离(UVPD)应用于在线液相色谱串联质谱仪(MS/MS)策略，以支持在疫苗佐剂开发过程中联合修饰的A类脂的复杂混合物的分析。利用Orbitrap质谱仪，在193 nm波长处对大肠杆菌、霍乱弧菌和铜绿假单胞菌脂类A的结构和碎片离子类型进行了分析。将UVPD产生的碎片离子与碰撞诱导解离(CID)和高能碰撞解离(HCD)产生的碎片离子与前驱电荷态进行了比较。UVPD提供了最广泛的碎片离子类型，包括酰链C-O、C-N和C-C键裂解以及糖苷C-O裂解和交叉环裂解，从而提供了对脂质A的最全面的结构分析。UVPD几乎不依赖于前体离子电荷状态，并且在确定包括酰链长度和组成在内的脂类A结构方面表现得最好，这使其优于基于碰撞的方法。将UVPD结合到LC-MS/MS方法中，用于分析组合工程大肠杆菌脂类A的复杂混合物中的一些结构变体。
Here we implement ultraviolet photodissociation (UVPD) in an online liquid chromatographic tandem mass spectrometry (MS/MS) strategy to support analysis of complex mixtures of lipid A combinatorially modified during development of vaccine adjuvants. UVPD mass spectrometry at 193 nm was utilized to characterize the structures and fragment ion types of lipid A from Escherichia coli, Vibrio cholerae, and Pseudomonas aeruginosa using an Orbitrap mass spectrometer. The fragment ions generated by UVPD were compared to those from collision induced dissociation (CID) and higher energy collision dissociation (HCD) with respect to the precursor charge state. UVPD afforded the widest array of fragment ion types including acyl chain C–O, C–N, and C–C bond cleavages and glycosidic C–O and cross ring cleavages, thus providing the most comprehensive structural analysis of the lipid A. UVPD exhibited virtually no dependence on precursor ion charge state and was best at determining lipid A structure including acyl chain length and composition, giving it an advantage over collision based methods. UVPD was incorporated into an LC–MS/MS methodology for the analysis of a number of structural variants in a complex mixture of combinatorially engineered Escherichia coli lipid A.
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DOI：
10.1016/j.jasms.2010.01.008
发表时间：
2010-05-01
影响因子：
3.2
作者：
Jones, Jace W.;Cohen, Ilana E.;Ernst, Robert K.
通讯作者：
Ernst, Robert K.
影响因子：
2.9
作者：
Kussak, A;Weintraub, A
通讯作者：
Weintraub, A
影响因子：
7.4
作者：
Ko, Byoung Joon;Brodbelt, Jennifer S.
通讯作者：
Brodbelt, Jennifer S.
DOI：
10.1016/j.jasms.2007.08.007
发表时间：
2007-11-01
影响因子：
3.2
作者：
Gabelica, Valerie;Rosu, Frederic;Dugourd, Philippe
通讯作者：
Dugourd, Philippe
影响因子：
2.3
作者：
Kilar, Aniko;Doernyei, Agnes;Kilar, Ferenc
通讯作者：
Kilar, Ferenc