Selection of rat hepatoma cells defective in hormone-regulated production of mouse mammary tumor virus RNA.
Selection of rat hepatoma cells defective in hormone-regulated production of mouse mammary tumor virus RNA.
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选择在激素调节的小鼠乳腺肿瘤病毒 RNA 生产中有缺陷的大鼠肝癌细胞。
DOI：
10.1073/pnas.78.7.4349
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发表时间：
1981
影响因子：
11.1
通讯作者：
Ringold,GM
中科院分区：
文献类型：
--
作者：
Grove,JR;Ringold,GM
我们以小鼠乳腺肿瘤病毒(MMTV)感染的大鼠肝癌细胞为模型系统，研究糖皮质激素的作用机制。克隆细胞系J2.17含有一个MMTV前病毒，可诱导酪氨酸转氨酶(酪氨酸氨基转移酶；L酪氨酸：2-氧戊二酸转氨酶，EC 2.6.1.5)、病毒核糖核酸和细胞表面病毒糖蛋白gp52对地塞米松的反应。使用荧光激活的细胞分选器和针对gp52的兔抗血清，我们选择了一个细胞群体，该细胞群体表现出激素介导的细胞表面gp52增加减少。对该群体中的14个克隆进行了病毒gp52、RNA和细胞TyrATase的诱导试验。这些检测结果表明，这些克隆对激素表现出不同的反应。其中一个克隆保留了TyrATase和gp52的野生型应答。6个无性系对这两个标记的反应协调减弱或取消。有7个克隆显示了对TyrATase的正常诱导，但对gp52的诱导减少或检测不到。这些克隆表现出MMTV RNA产量的减少，因此可能代表了一类独特的在调节MMTV基因表达方面存在缺陷的变异体。
We have been studying the mechanism of glucocorticoid hormone action by using mouse mammary tumor virus (MMTV)-infected rat hepatoma cells as a model system. J2.17, a clonal cell line that contains one MMTV provirus, induces tyrosine aminotransferase (TyrATase; L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5), viral RNA, and the cell surface viral glycoprotein gp52 in response to dexamethasone. Using a fluorescence-activated cell sorter and a rabbit antiserum directed against gp52, we selected a cell population that displays a reduced hormone-mediated increase in cell surface gp52. Fourteen clones of this population were assayed for induction of viral gp52 and RNA and of cellular TyrATase. The results of these assays revealed that the clones display a variety of responses to hormone. One clone has retained wild-type responses of both TyrATase and gp52. Six clones exhibit coordinately reduced or abolished responses of both markers. Seven clones show normal induction of TyrATase but reduced or undetectable induction of gp52. These latter clones exhibit reduced production of MMTV RNA and thus may represent a unique class of variants defective in the regulation of MMTV gene expression.
类固醇抵抗机制：淋巴瘤细胞中糖皮质激素受体缺陷。
DOI：
--
发表时间：
1972
期刊：
Nature: New biology
影响因子：
--
作者：
W. Rosenau;J. Baxter;G. Rousseau;G. Tomkins
通讯作者：
G. Tomkins