Solid-state NMR studies for the development of non-woven biomaterials based on silk fibroin and polyurethane
Solid-state NMR studies for the development of non-woven biomaterials based on silk fibroin and polyurethane
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用于开发基于丝素蛋白和聚氨酯的非织造生物材料的固态核磁共振研究
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y. Nakazawa
中科院分区：
文献类型：
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作者：
Chikako T. Nakazawa;Akira Higuchi;A. Asano;T. Kameda;D. Aytemiz;Y. Nakazawa
丝素蛋白（SF）具有多种有利于组织工程的特性。然而，在某些情况下必须改变机械性能。例如，循环系统等软组织比组织工程材料更有弹性。聚合物共混物的开发是一种简单的方法，有可能提供具有扩展有用性能的材料。在这项研究中，我们利用聚合物溶液开发了一种非织造 SF 和热塑性聚氨酯共混片材。使用固态核磁共振（NMR）方法评估共混片材的结构和混溶性。在 13C 交叉偏振和魔角旋转 NMR 谱中，纯样品和混合样品之间没有观察到峰移。使用实验室框架中的质子自旋晶格弛豫时间 (T1H) 研究样品的混溶性。 T1H测量清楚地表明SF和Pellethane的分子链以数十纳米的距离紧密存在。聚合物共混物的开发是一种简单的方法，有可能提供具有扩展有用性能的材料。在这里，尽管 SF 保持了强的 β 片晶体结构，但观察到 SF 和 Pellethane 彼此高度混溶。该结果表明，SF/Pellethane 共混片材具有源自 SF 的强度和源自 Pellethane 的弹性。因此，这种共混片材应该是一种良好的软组织工程医用材料。
Silk fibroin (SF) possesses several characteristics that are favorable for tissue engineering. However, the mechanical properties must be modified in some cases. For instance, soft tissues such as those of the circulatory system are more elastic than tissue-engineered materials. The development of polymer blends is a simple method with the potential to provide materials with extended useful properties. In this study, we developed a non-woven SF and thermoplastic polyurethane-blend sheet from a polymer solution. The structure and miscibility of the blend sheet was evaluated using solid-state nuclear magnetic resonance (NMR) methods. In the 13C cross-polarization and magic angle spinning NMR spectra, no peak shift was observed between the pure and blended samples. The miscibility of the sample was investigated using proton spin-lattice relaxation times in the laboratory frame (T1H). The T1H measurement clearly revealed that the molecular chains of SF and Pellethane exist in close proximity of several tens of nanometers. The development of polymer blends is a simple method with the potential to provide materials with extended useful properties. Here, the SF and Pellethane were observed to be highly miscible with each other even though SF maintained the strong β-sheet crystal structure. This result suggests that SF/Pellethane-blended sheets possess strength that is derived from SF and elasticity that is derived from Pellethane. Therefore, this blended sheet should be a good medical material for soft tissue engineering.
影响因子：
2.7
作者：
Ravi S;Chaikof EL
通讯作者：
Chaikof EL