Retinal cone photoreceptors of the deer mouse Peromyscus maniculatus: development, topography, opsin expression and spectral tuning.
Retinal cone photoreceptors of the deer mouse Peromyscus maniculatus: development, topography, opsin expression and spectral tuning.
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DOI：
10.1371/journal.pone.0080910
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peichl L
中科院分区：
文献类型：
--
作者：
Arbogast P;Glösmann M;Peichl L
在夜间鹿小鼠，Peromyscus maniculatus，使用色素（野生型）和白化病动物的视网膜中进行感光特性的定量分析。目的是建立鹿鼠是否是一个更合适的模式物种比家鼠感光器的研究，以及眼皮肤白化病是否影响其感光性能。在视网膜平片中，通过视蛋白免疫染色鉴定视锥光感受器，并确定它们的数量、光谱类型和在视网膜上的分布。使用微分干涉对比显微镜计数视杆细胞。着色的Maniculatus具有以杆为主的视网膜，其中杆密度为约450.000/mm 2，锥密度为3000 - 6500/mm 2。存在两种视锥视蛋白，短波敏感（S）和中长波敏感（M），并在不同的视锥类型中表达。S视蛋白基因的部分测序强烈支持S视锥视色素的UV敏感性。S型视锥细胞占视锥细胞的5-15%。与家鼠不同，S和M视锥的分布不具有背腹梯度，并且两种视蛋白在单个视锥中的共表达是例外的（<2%的视锥）。在白化病的P. maniculatus中，杆密度减少了约40%（270.000/mm 2）。总的来说，视锥密度和仅表达S视蛋白的视锥密度与着色的Maniculatus没有显著差异。然而，在白化病视网膜中，S视蛋白与M视蛋白在60-90%的视锥细胞中共表达，因此仅表达M视蛋白的视锥细胞群显著减少至5- 25%。总之，鹿鼠视锥特性在很大程度上符合一般的哺乳动物模式，因此鹿鼠可能比家鼠更适合于某些基本的视锥特性的研究，包括白化对视锥视蛋白表达的影响。
A quantitative analysis of photoreceptor properties was performed in the retina of the nocturnal deer mouse, Peromyscus maniculatus, using pigmented (wildtype) and albino animals. The aim was to establish whether the deer mouse is a more suitable model species than the house mouse for photoreceptor studies, and whether oculocutaneous albinism affects its photoreceptor properties. In retinal flatmounts, cone photoreceptors were identified by opsin immunostaining, and their numbers, spectral types, and distributions across the retina were determined. Rod photoreceptors were counted using differential interference contrast microscopy. Pigmented P. maniculatus have a rod-dominated retina with rod densities of about 450.000/mm2 and cone densities of 3000 - 6500/mm2. Two cone opsins, shortwave sensitive (S) and middle-to-longwave sensitive (M), are present and expressed in distinct cone types. Partial sequencing of the S opsin gene strongly supports UV sensitivity of the S cone visual pigment. The S cones constitute a 5-15% minority of the cones. Different from house mouse, S and M cone distributions do not have dorsoventral gradients, and coexpression of both opsins in single cones is exceptional (<2% of the cones). In albino P. maniculatus, rod densities are reduced by approximately 40% (270.000/mm2). Overall, cone density and the density of cones exclusively expressing S opsin are not significantly different from pigmented P. maniculatus. However, in albino retinas S opsin is coexpressed with M opsin in 60-90% of the cones and therefore the population of cones expressing only M opsin is significantly reduced to 5-25%. In conclusion, deer mouse cone properties largely conform to the general mammalian pattern, hence the deer mouse may be better suited than the house mouse for the study of certain basic cone properties, including the effects of albinism on cone opsin expression.
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影响因子：
2.5
作者：
Moritz, Gillian L.;Lim, Norman T. -L.;Neitz, Maureen;Peichl, Leo;Dominy, Nathaniel J.
通讯作者：
Dominy, Nathaniel J.
影响因子：
3.7
作者：
通讯作者：
--
DOI：
10.1007/s00359-003-0408-0
发表时间：
2003-05-01
影响因子：
2.1
作者：
Jacobs, GH;Calderone, JB;Williams, GA
通讯作者：
Williams, GA
影响因子：
3.4
作者：
JEFFERY, G;DARLING, K;WHITMORE, A
通讯作者：
WHITMORE, A
影响因子：
1.9
作者：
de Farias Rocha, Fernando Allan;Ahnelt, Peter K.;De Lima, Silene Maria A.
通讯作者：
De Lima, Silene Maria A.