Metastasis‐associated Mts1 (S100A4) protein in the developing and adult central nervous system
Metastasis‐associated Mts1 (S100A4) protein in the developing and adult central nervous system
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发育中和成人中枢神经系统中的转移相关 Mts1 (S100A4) 蛋白
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
E. Kozlova
中科院分区：
文献类型：
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作者：
F. Åberg;E. Kozlova
我们最近发现脊髓中的白质星形胶质细胞组成性地表达Mts1 （S100A4）蛋白的免疫反应性，并且这种表达在坐骨神经或背根损伤后同侧上调。本研究研究了Mts1在大鼠中枢神经系统（CNS）中的表达模式。我们发现Mts1免疫反应性存在于髓鞘束，如嗅觉束、视神经、胼胝体、内囊、纤维和脊髓索，但不存在于小脑白质。Mts1‐免疫反应（IR）细胞一致呈星形胶质细胞（胶质纤维酸性蛋白阳性）。除了髓鞘束外，Mts1免疫反应性也存在于少数无髓鞘或髓鞘较差的区域，如脑垂体、嗅球和侧脑室周围。根据位置，Mts1‐IR星形胶质细胞被区分为3组：1)星形胶质细胞位于中枢神经系统表面，即靠近脑脊液，垂直于轴突束排列；2)星形胶质细胞平行于轴突束并插入轴突束之间；3)侧脑室周围和嗅球内的星形胶质细胞群。我们进一步通过Mts1和髓鞘碱性蛋白（myelin basic protein， MBP）联合染色分析Mts1免疫反应性与CNS纤维束发育的关系。新生髓鞘区出现Mts1免疫反应性。在胼胝体和视神经束发育过程中，Mts1免疫反应性主要集中在髓鞘形成的前沿。发育表达模式提示Mts1‐IR星形胶质细胞在髓鞘纤维束成熟中的作用。成熟中枢神经系统中Mts1优先定位于基底下区，表明Mts1参与星形胶质细胞介导的中枢神经系统-脑脊液交换。[j]中华神经科杂志，2004,24(4):369 - 382。©2000 Wiley‐Liss, Inc。
We have found recently that white matter astrocytes in the spinal cord constitutively express immunoreactivity for Mts1 (S100A4) protein and that this expression is up‐regulated ipsilaterally after sciatic nerve or dorsal root injury. Here, we have studied the expression pattern of Mts1 throughout the rat central nervous system (CNS). We found Mts1 immunoreactivity in myelinated tracts such as the olfactory tract, optic nerve, corpus callosum, internal capsule, fimbria, and spinal cord funiculi but not in cerebellar white matter. Mts1‐immunoreactive (IR) cells were consistently astrocytic (glial fibrillary acidic protein positive). In addition to myelinated tracts, Mts1 immunoreactivity was also present in a few nonmyelinated or poorly myelinated areas, such as pituitary gland, olfactory bulb, and around the lateral ventricle. Based on location, three Mts1‐IR astrocyte groups were distinguished: 1) astrocytes at the surfaces of the CNS, i.e., adjacent to the cerebrospinal fluid, organized perpendicularly to the bundles of axonal tracts; 2) astrocytes located in parallel to, and inserted between, axonal bundles; and 3) clusters of astrocytes around the lateral ventricle and in the olfactory bulb. We further analyzed the relationship between Mts1 immunoreactivity and the development of CNS fiber tracts by combining staining for Mts1 and myelin basic protein (MBP). Mts1 immunoreactivity appeared postnatally in recently myelinated areas. During the development of corpus callosum and the optic tract, Mts1 immunoreactivity was concentrated at the frontier of myelination. The developmental expression pattern suggests a role of Mts1‐IR astrocytes in the maturation of myelinated fiber tracts. The preferential localization of Mts1 to the subpial region in the mature CNS suggests that Mts1 participates in astrocyte‐mediated CNS‐cerebrospinal fluid exchange. J. Comp. Neurol. 424:269–282, 2000. © 2000 Wiley‐Liss, Inc.
影响因子：
8
作者：
Heide L. Ford;Sayeeda Zain
通讯作者：
Heide L. Ford;Sayeeda Zain
影响因子：
2
作者：
G. Sherbet;M. S. Lakshmi
通讯作者：
G. Sherbet;M. S. Lakshmi
影响因子：
2.9
作者：
Ford, HL;Silver, DL;Zain, SB
通讯作者：
Zain, SB