Expression of recombinant Schistosoma japonicum protein rSjE16 and its effects on LX-2 cells in vitro.
Expression of recombinant Schistosoma japonicum protein rSjE16 and its effects on LX-2 cells in vitro.
复制标题
重组日本血吸虫蛋白rSjE16的体外表达及其对LX-2细胞的影响
DOI：
10.1177/0300060520972228
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
Gao W;Peng W;Ji X;Zhu D;Chen J;Feng J;Yu Y;Duan L;Duan Y
目的肝星状细胞（HSCs）的活化是血吸虫诱导肝纤维化的关键事件。先前的研究表明，日本血吸虫卵的可溶性抗原和重组P40蛋白抑制HSC的激活。在本研究中，我们观察了日本血吸虫重组(r)SjE16蛋白对hsc的直接作用。方法采用生物信息学方法对SjE16基因序列进行分析。采用western blotting、定量PCR、MTT等方法观察rSjE16对hsc的影响。结果SjE16蛋白无信号肽，无跨膜区。rSjE16显著抑制LX-2细胞α-平滑肌肌动蛋白和I型胶原蛋白的表达水平。rSjE16还显著提高了LX-2细胞中白细胞介素(IL)-6和IL-8的表达水平，增强了基质金属蛋白酶(MMP)-2、MMP-9和过氧化物酶体增殖物激活受体-γ的表达。rSjE16对LX-2细胞活力无抑制作用。结论rSjE16可能通过复杂的分子机制参与HSC的活化过程，其机制有待进一步研究。
Objective The activation of hepatic stellate cells (HSCs) is a key event in schistosome-induced liver fibrosis. Previous studies have shown that soluble egg antigens and the recombinant P40 protein from Schistosoma japonicum eggs inhibit HSC activation. In the present study, we observed the direct effect of the S. japonicum recombinant (r)SjE16 protein on HSCs. Methods The sequence of SjE16 was analyzed by bioinformatics. Then western blotting, quantitative PCR, and MTT assays were performed to observe the effects of rSjE16 on HSCs. Results The SjE16 protein has no signal peptide or transmembrane region. rSjE16 significantly inhibited expression levels of α-smooth muscle actin and collagen I protein in LX-2 cells. rSjE16 also significantly increased the expression levels of interleukin (IL)-6 and IL-8, and enhanced the expression of matrix metalloproteinase (MMP)-2, MMP-9, and peroxisome proliferator-activated receptor-γ in LX-2 cells. LX-2 cell viability was not inhibited by rSjE16. Conclusion rSjE16 may be involved in the progression of HSC activation via a complex molecular mechanism, which requires further study to fully understand.
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影响因子：
7.3
作者：
Hams E;Aviello G;Fallon PG
通讯作者：
Fallon PG
SjE16.7激活巨噬细胞并促进日本血吸虫卵诱导的肉芽肿发展
影响因子：
2.7
作者：
Fang, Yan;Wu, Chenyun;Wang, Zhaojun
通讯作者：
Wang, Zhaojun
日本血吸虫可溶性卵抗原诱导肝星状细胞凋亡并抑制肝星状细胞活化：可能的分子机制
影响因子：
4
作者：
Duan, Yinong;Gu, Xijuan;He, Xingxin
通讯作者：
He, Xingxin
影响因子：
2.1
作者：
Anthony, Barrie;Mathieson, William;Allen, Jeremy
通讯作者：
Allen, Jeremy
DOI：
10.1111/j.1440-1746.2006.04586.x
发表时间：
2006-10-01
影响因子：
4.1
作者：
Han, Yuan-Ping
通讯作者：
Han, Yuan-Ping