The COP1 Target SHI-RELATED SEQUENCE5 Directly Activates Photomorphogenesis-Promoting Genes
The COP1 Target SHI-RELATED SEQUENCE5 Directly Activates Photomorphogenesis-Promoting Genes
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COP1 靶标 SHI 相关序列 5 直接激活光形态发生促进基因
DOI：
10.1105/tpc.18.00455
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Lu Ying-Tang
中科院分区：
文献类型：
--
作者：
Yuan Ting-Ting;Xu Heng-Hao;Zhang Qing;Zhang Lin-Yu;Lu Ying-Tang
蓝光诱导的SRS5通过直接激活促进光形态建成的基因如HY5、BBX21和BBX22来促进光形态建成，而COP1直接针对SRS5在黑暗中降解。植物幼苗在黑暗和光照中经历不同的发育过程。一些基因，包括延长的HYPOCOTYL5(HY5)、B-box PROTEIN21(BBX21)和BBX22，已经被鉴定为拟南芥的光形态建成促进因子；然而，这些基因的过度表达并不能在黑暗中诱导光形态建成。利用激活标签的方法，我们鉴定了SRS5ox，它在雌激素诱导下过表达SHI相关的SEQUENCE5(SRS5)。SRS5在SRS5ox和Pro35S：SRS5-GFP中的过表达导致在黑暗中的结构性光形态建成表型，而在srs5-2突变体中SRS5的功能丧失导致在光照下产生较长的下胚轴。这表明SRS5是光形态建成的正调控因子。此外，SRS5通过直接与光形态建成促进基因的启动子HY5、BBX21和BBX22结合并激活它们的表达来促进光形态建成，从而影响下游光信号基因的表达。这些数据表明，SRS5在光形态建成促进基因的上调中起作用。此外，在幼苗光形态建成过程中起中心抑制作用的结构性光敏蛋白1(COP1)直接泛素化SRS5，促进其在黑暗中的降解。综上所述，我们的结果表明，SRS5直接激活了下游基因HY5、BBX21和BBX22的表达，是COP1介导的拟南芥降解的靶标。
Blue light-induced SRS5 promotes photomorphogenesis by directly activating photomorphogenesis-promoting genes such as HY5, BBX21 and BBX22, but COP1 directly targets SRS5 for degradation in the dark. Plant seedlings undergo distinct developmental processes in the dark and in the light. Several genes, including ELONGATED HYPOCOTYL5 (HY5), B-BOX PROTEIN21 (BBX21), and BBX22, have been identified as photomorphogenesis-promoting factors in Arabidopsis thaliana; however, the overexpression of these genes does not induce photomorphogenesis in the dark. Using an activation-tagging approach, we identified SRS5ox, which overexpresses SHI-RELATED SEQUENCE5 (SRS5) following induction with estradiol. SRS5 overexpression in SRS5ox and Pro35S:SRS5-GFP seedlings results in a constitutive photomorphogenesis phenotype in the dark, whereas SRS5 loss of function in the srs5-2 mutant results in long hypocotyls in the light. This indicates that SRS5 is a positive regulator of photomorphogenesis. Furthermore, SRS5 promotes photomorphogenesis by directly binding to the promoters of photomorphogenesis-promoting genes, such as HY5, BBX21, and BBX22, and activating their expression, thus affecting the expression of downstream light-signaling genes. These data indicate that SRS5 acts in the upregulation of photomorphogenesis-promoting genes. In addition, CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), which plays a central repressive role in seedling photomorphogenesis, directly ubiquitinates SRS5, promoting its degradation in the dark. Taken together, our results demonstrate that SRS5 directly activates the expression of downstream genes HY5, BBX21, and BBX22 and is a target of COP1-mediated degradation in Arabidopsis.
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影响因子：
4
作者：
Xi Huang;X. Ouyang;X. Deng;X. Deng;Wang;Xiangdong Fu;J. Kyozuka
通讯作者：
Xi Huang;X. Ouyang;X. Deng;X. Deng;Wang;Xiangdong Fu;J. Kyozuka
影响因子：
11.6
作者：
Sang, Y;Li, QH;Yang, HQ
通讯作者：
Yang, HQ
DOI：
10.1104/pp.114.3.779
发表时间：
1997-07
期刊：
--
影响因子：
--
作者：
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通讯作者：
A. Arnim;M. Osterlund;S. Kwok;X. Deng
影响因子：
7.4
作者：
Li, Juan;Xu, Heng-Hao;Lu, Ying-Tang
通讯作者：
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影响因子：
5.1
作者：
Jing Wang;Da-wei Yan;Ting-Ting Yuan-Ting;Xiang Gao;Ying‐Tang Lu
通讯作者：
Jing Wang;Da-wei Yan;Ting-Ting Yuan-Ting;Xiang Gao;Ying‐Tang Lu