Total flavonoids from Astragalus alleviate endothelial dysfunction by activating the Akt/eNOS pathway.
Total flavonoids from Astragalus alleviate endothelial dysfunction by activating the Akt/eNOS pathway.
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黄芪总黄酮通过激活 Akt/eNOS 通路缓解内皮功能障碍
DOI：
10.1177/0300060517717358
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Mao Y
中科院分区：
文献类型：
--
作者：
Liu Q;Zhang L;Shan Q;Ding Y;Zhang Z;Zhu M;Mao Y
目的探讨黄芪总黄酮（TFA）的扩血管和保护内皮作用及其机制。方法采用离体大鼠肠系膜上上级动脉环肌电描记仪测定TFA的舒张血管活性。采用细胞计数试剂盒-8检测缺氧或常氧处理后，加入或不加入TFA的原代人脐静脉内皮细胞（HUVEC）的活力。Western blotting检测Akt、P-Akt、eNOS、P-eNOS、Erk、P-Erk、Bcl-2和Bax的表达。结果TFA对大鼠肠系膜上上级动脉环具有浓度依赖性舒张作用，对正常和氯化钾预收缩的动脉环无明显舒张作用。TFA在常氧条件下不影响HUVEC的活力，但在低氧条件下，在1至30 µg/mL的浓度范围内显著促进细胞增殖。TFA可显著增加缺氧条件下血管内皮细胞P-Akt/Akt和P-eNOS/eNOS的比值，但不改变P-Erk/Erk和Bcl-2/Bax的比值。结论TFA可能通过Akt/eNOS信号通路发挥血管舒张和内皮保护作用。
Objective To investigate the vasodilative and endothelial-protective effects and the underlying mechanisms of total flavonoids from Astragalus (TFA). Methods The vasodilative activities of TFA were measured with a myograph ex vivo using rat superior mesenteric arterial rings. The primary human umbilical vein endothelial cell (HUVEC) viabilities were assayed using the cell counting kit-8 after hypoxia or normoxia treatment with or without TFA. Akt, P-Akt, eNOS, P-eNOS, Erk, P-Erk, Bcl-2 and Bax expression were analyzed using western blotting. Results TFA showed concentration-dependent vasodilative effects on rat superior mesenteric arterial rings, but had no effects on normal or potassium chloride precontracted arterial rings. TFA did not affect HUVEC viabilities in normoxia, but dramatically promoted cell proliferation in the concentration range of 1 to 30 µg/mL under hypoxia. Moreover, TFA significantly increased the ratios of P-Akt/Akt and P-eNOS/eNOS in vascular endothelial cells under hypoxic conditions, but did not change the P-Erk/Erk or Bcl-2/Bax ratios. Conclusions TFA might exhibit vasorelaxant and endothelial-protective effects via the Akt/eNOS signaling pathway.
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