Investigation of effects and mechanisms of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
Investigation of effects and mechanisms of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
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DOI：
10.1155/2012/209843
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发表时间：
2012
影响因子：
--
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Zhang D;Zhuang Y;Pan J;Wang H;Li H;Yu Y;Wang D
黄酮类化合物存在于植物的大部分部位，已被证明具有抗癌、抗炎、抗菌、抗病毒、免疫刺激等多种生物活性。现有资料表明，黄芪总黄酮（TFA）可以为生物系统提供抗损伤能力，并具有抗突变、抑制动脉粥样硬化等生物学作用。本研究通过一系列技术，包括增殖 (MTT)、PI 染色、Annexin V/PI 双染色和 RT-PCR，研究了 TFA 和毛蕊异黄酮（从 TFA 中分离出的化合物）对人红白血病细胞系 K562 细胞凋亡诱导和细胞周期的影响。实验数据表明，TFA和毛蕊异黄酮能抑制K562细胞的增殖。 TFA和毛蕊异黄酮的50%抑制浓度分别为98.63μg/mL和130.32μg/mL。然而，TFA和毛蕊异黄酮不能诱导K562细胞凋亡，但可以增加G0/G1期细胞的数量。 TFA和毛蕊异黄酮处理后K562细胞中cyclin D1 mRNA的水平下降。该研究为黄芪总黄酮和毛蕊异黄酮对人红白血病细胞的作用机制提供了新的见解。
Flavonoids are found in most parts of plants and have been shown to have multiple biological activities such as anticancer, anti-inflammation, antibacteria, antivirus, and immune-stimulation. Existing data showed that the total flavonoids of Astragalus (TFA) can provide biological system with resistance to injury and can possess antimutagenic, atherosclerotic inhibition, and other biological effects. This study investigated the effects of TFA and calycosin (a compound isolated from TFA), on apoptosis induction, and cell cycle of human erythroleukemia cell line K562 by an array of techniques, including proliferation (MTT), PI staining, Annexin V/PI double staining, and RT-PCR. The experimental data showed that TFA and calycosin could inhibit the proliferation of K562 cells. The 50% inhibiting concentrations of TFA and calycosin were 98.63 μ g/mL and 130.32 μ g/mL, respectively. However, TFA and calycosin could not induce apoptosis in K562 cells, but could increase the number of the cells in the G0/G1 phase. The level of cyclin D1 mRNA in K562 cells decreased after the treatment with TFA and calycosin. This study provides new insights into the functional mechanism of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
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影响因子：
--
作者：
Kadowaki N;Kitawaki T
通讯作者：
Kitawaki T
影响因子：
2.9
作者：
Suttorp, Meinolf;Eckardt, Louise;Millot, Frederic
通讯作者：
Millot, Frederic
影响因子：
5.4
作者：
Siriwatanametanon, Nisarat;Fiebich, Bernd L.;Heinrich, Michael
通讯作者：
Heinrich, Michael
研究星形素的总类黄酮对动脉粥样硬化形成和潜在机制的影响。
影响因子：
--
作者：
Wang D;Zhuang Y;Tian Y;Thomas GN;Ying M;Tomlinson B
通讯作者：
Tomlinson B
影响因子：
5.2
作者：
Israels, ED;Israels, LG
通讯作者：
Israels, LG