Pathoconnectome Analysis of Müller Cells in Early Retinal Remodeling.
Pathoconnectome Analysis of Müller Cells in Early Retinal Remodeling.
复制标题
早期视网膜重塑中 Müller 细胞的病理连接组分析。
DOI：
10.1007/978-3-030-27378-1_60
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发表时间：
2019
影响因子：
--
通讯作者：
Jones,BryanW
中科院分区：
文献类型：
--
作者：
Pfeiffer,RebeccaL;Anderson,JamesR;Emrich,DanielP;Dahal,Jeebika;Sigulinsky,CrystalL;Morrison,HopeAB;Yang,Jia-Hui;Watt,CarlB;Rapp,KevinD;Kondo,Mineo;Terasaki,Hiroko;Garcia,JessicaC;Marc,RobertE;Jones,BryanW
神经胶质细胞在神经功能中发挥重要作用，包括但不限于氨基酸回收、离子稳态、葡萄糖代谢和废物清除。在视网膜变性和随后的视网膜重塑过程中，穆勒细胞（MC）是第一个因应激而表现出代谢和形态变化的细胞。视网膜感光细胞变性时，MCs 的代谢变化混乱地进展；然而，这些改变与神经元应激、突触维持或胶质细胞-胶质细胞相互作用之间的关系目前尚不清楚。这里描述的工作从早期视网膜重塑视网膜病理连接组 1 (RPC1) 的病理连接组重建 MC，并探索邻近神经元和胶质细胞背景下 MC 结构和代谢表型之间的关系。在这里，我们发现细胞间和细胞内锇强度的变化、MC小分子代谢含量的变化以及神经胶质末足的形态变化。 RPC1 提供了一个框架，通过神经元和神经胶质细胞的超微结构重建来分析早期视网膜重塑中的这些关系。这些重建通过计算分子表型分析 (CMP) 进行定量代谢物标记，使我们能够以前所未有的分辨率和灵敏度评估早期视网膜变性中的神经胶质细胞相互作用。
Glia play important roles in neural function, including but not limited to amino acid recycling, ion homeostasis, glucose metabolism, and waste removal. During retinal degeneration and subsequent retinal remodeling, Müller cells (MCs) are the first cells to show metabolic and morphological alterations in response to stress. Metabolic alterations in MCs chaotically progress in retina undergoing photoreceptor degeneration; however, what relationship these alterations have with neuronal stress, synapse maintenance, or glia-glia interactions is currently unknown. The work described here reconstructs a MC from a pathoconnectome of early retinal remodeling retinal pathoconnectome 1 (RPC1) and explores relationships between MC structural and metabolic phenotypes in the context of neighboring neurons and glia. Here we find variations in intensity of osmication inter- and intracellularly, variation in small molecule metabolic content of MCs, as well as morphological alterations of glial endfeet. RPC1 provides a framework to analyze these relationships in early retinal remodeling through ultrastructural reconstructions of both neurons and glia. These reconstructions, informed by quantitative metabolite labeling via computational molecular phenotyping (CMP), allow us to evaluate neural-glial interactions in early retinal degeneration with unprecedented resolution and sensitivity.
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影响因子：
2.2
作者：
James R. Anderson;B. Jones;C. Watt;M. V. Shaw;Jia-Hui Yang;D. DeMill;J. S. Lauritzen;Yanhua Lin;K. Rapp;D. Mastronarde;Pavel A. Koshevoy;B. Grimm;T. Tasdizen;R. Whitaker;R. Marc
通讯作者：
James R. Anderson;B. Jones;C. Watt;M. V. Shaw;Jia-Hui Yang;D. DeMill;J. S. Lauritzen;Yanhua Lin;K. Rapp;D. Mastronarde;Pavel A. Koshevoy;B. Grimm;T. Tasdizen;R. Whitaker;R. Marc
影响因子：
4.2
作者：
Fariss, RN;Li, ZY;Milam, AH
通讯作者：
Milam, AH
分离的猫视网膜中的氨基酸特征。
DOI：
--
发表时间：
1998
期刊：
Investigative ophthalmology & visual science.
影响因子：
--
作者：
Marc,RE;Murry,RF;Fisher,SK;Linberg,KA;Lewis,GP
通讯作者：
Lewis,GP
影响因子：
3.4
作者：
Jones, B. W.;Pfeiffer, R. L.;Ferrell, W. D.;Watt, C. B.;Marmor, M.;Marc, R. E.
通讯作者：
Marc, R. E.
影响因子：
3.4
作者：
Pfeiffer, Rebecca L.;Marc, Robert E.;Kondo, Mineo;Terasaki, Hiroko;Jones, Bryan W.
通讯作者：
Jones, Bryan W.