Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14.
Arabidopsis thaliana DOF6 negatively affects germination in non-after-ripened seeds and interacts with TCP14.
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DOI：
10.1093/jxb/err388
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发表时间：
2012-03
影响因子：
6.9
通讯作者：
Oñate-Sánchez L
中科院分区：
文献类型：
--
作者：
Rueda-Romero P;Barrero-Sicilia C;Gómez-Cadenas A;Carbonero P;Oñate-Sánchez L
种子休眠可防止种子在不利于植物生长和发育的环境条件下发芽，构成进化优势。干燥储存，也称为后熟，通过尚不清楚的机制逐渐减少种子休眠。影响种子萌发的拟南芥 DOF 转录因子基因 (DOF6) 已得到表征。该基因的转录水平在干燥种子中积累，并在后熟过程中以及种子吸胀过程中逐渐衰减。虽然 DOF6 的组成型过度表达会导致植物生长异常和不育，但其在种子吸胀时诱导的过度表达会引发发芽延迟、脱落酸 (ABA) 过敏表型以及 ABA 生物合成基因 ABA1 和 ABA 相关胁迫基因的表达增加。尽管存在 DOF6 诱导的过度表达，但野生型发芽和基因表达在种子后熟过程中逐渐恢复。 DOF6被发现在酵母双杂交系统和植物中与TCP14相互作用，TCP14是先前描述的种子萌发的正调节因子。 ABA1 和 ABA 相关应激基因的表达在 tcp14 敲除突变体中也得到增强。总而言之，这些结果表明 DOF6 对种子萌发产生负面影响，并反对 TCP14 在调节一组特定 ABA 相关基因中的功能。
Seed dormancy prevents seeds from germinating under environmental conditions unfavourable for plant growth and development and constitutes an evolutionary advantage. Dry storage, also known as after-ripening, gradually decreases seed dormancy by mechanisms not well understood. An Arabidopsis thaliana DOF transcription factor gene (DOF6) affecting seed germination has been characterized. The transcript levels of this gene accumulate in dry seeds and decay gradually during after-ripening and also upon seed imbibition. While constitutive over-expression of DOF6 produced aberrant growth and sterility in the plant, its over-expression induced upon seed imbibition triggered delayed germination, abscisic acid (ABA)-hypersensitive phenotypes and increased expression of the ABA biosynthetic gene ABA1 and ABA-related stress genes. Wild-type germination and gene expression were gradually restored during seed after-ripening, despite of DOF6-induced over-expression. DOF6 was found to interact in a yeast two-hybrid system and in planta with TCP14, a previously described positive regulator of seed germination. The expression of ABA1 and ABA-related stress genes was also enhanced in tcp14 knock-out mutants. Taken together, these results indicate that DOF6 negatively affects seed germination and opposes TCP14 function in the regulation of a specific set of ABA-related genes.
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影响因子：
7.4
作者：
Debeaujon, I;Koornneef, M
通讯作者：
Koornneef, M
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.2
作者：
Gabriele, Stefano;Rizza, Annalisa;Vittorioso, Paola
通讯作者：
Vittorioso, Paola
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Groszmann, Michael;Paicu, Teodora;Smyth, David R.
通讯作者：
Smyth, David R.